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Strain: SD-Pde6bem1Baek

Symbol: SD-Pde6bem1Baek
Strain: SD-Pde6bem1Baek
Substrain: Baek
RGD ID: 42721977
Citation ID: RRID:RGD_42721977
Ontology ID: RS:0004863
Alleles: Pde6bem1Baek
Also Known As: SD-Pde6b^[em1Baek]
Type: mutant
Available Source: Not Available
Origination: Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea
Description: Cpf1 mRNA (50 ng per uL) and CRISPR RNA (100 ng per uL) were co-microinjected into the cytoplasm of pronuclear stage embryos; surviving embryos were transplanted into the oviducts of pseudo-pregnant surrogate mothers to generate live animals. A Pde6b-mutant rat line with an 11-bp deletion in exon 1 was established. Western blot analysis confirmed deficiency of Pde6b protein in the homozygous mutant retina, indicating successful generation of Pde6b-deficient rats with Cpf1-mediated gene targeting.
Last Known Status: Unknown
Position
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.2141,323,310 - 1,366,450RGD_MAPPER_PIPELINEmRatBN7.2
Rnor_6.0142,328,690 - 2,371,913RGD_MAPPER_PIPELINERnor6.0
Rnor_5.0142,327,104 - 2,370,811RGD_MAPPER_PIPELINERnor5.0
RGSC_v3.4141,871,037 - 1,914,170RGD_MAPPER_PIPELINERGSC3.4





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References

References - curated
# Reference Title Reference Citation
1. Development of a Pde6b Gene Knockout Rat Model for Studies of Degenerative Retinal Diseases. Yeo JH, etal., Invest Ophthalmol Vis Sci. 2019 Apr 1;60(5):1519-1526. doi: 10.1167/iovs.18-25556.

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