Strain Report - Rat Genome Database

Name: WI- Nanos3 em1(tdTomato)Nips
Creator: Rat Genome Database
License: Creative Commons CC BY 4.0
Keywords: Rat Gene RGD Genome

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Strain Registration

General

Strain: WI-Nanos3^{em1(tdTomato)Nips}

Symbol:

WI-Nanos3^{em1(tdTomato)Nips}

Strain:

WI-Nanos3^{em1(tdTomato)}

Substrain:

Nips

RGD ID:

401795481

Citation ID:

RRID:RGD_401795481

Ontology ID:

RS:0005295

Alleles:

Nanos3^{em1(tdTomato)Nips}

Also Known As:

WI-Nanos3^[em1(tdTomato)Nips]

Type:

mutant

Available Source:

Section of Mammalian Transgenesis Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki Aichi 444-8787, Japan. or Division of Mammalian Embryology, Center for Stem Cell Biology and Regenerative Medicine, The Institute for Medical Science, The University of Tokyo, Tokyo108-8639, Japan.

Description:

The targeting vector was designed to replace the stop codon of Nanos3 with T2A-2xtdTomato. The adeno-associated virus carrying the targeting vector was infected to Crlj:WI (RGD ID: 2312504) rat 1 cell zygotes followed by the introduction of CRISPR/Cas9 ribonucleoprotein complex by electroporation. After incubation overnight, the zygotes were transferred into oviducts of pseudo-pregnant rats. The pups were judged correct gene-targeting by genomic PCR. These rat strains are being maintained by crossing the founder rats with Crlj:WI rats. The tdTomato fluorescence faithfully label Nanos3 expressing cells.

Genetic Status:

Heterozygous

Last Known Status:

Cryopreserved Embryo; Cryopreserved Sperm (as of 2023-09-11)

Position

Rat Assembly	Chr	Position (strand)	Source	JBrowse
mRatBN7.2	19	23,978,465 - 23,988,933	RGD_MAPPER_PIPELINE	mRatBN7.2
Rnor_6.0	19	25,284,907 - 25,288,680	RGD_MAPPER_PIPELINE	Rnor6.0
Rnor_5.0	19	36,262,007 - 36,265,766	RGD_MAPPER_PIPELINE	Rnor5.0
RGSC_v3.4	19	25,669,353 - 25,672,779	RGD_MAPPER_PIPELINE	RGSC3.4

Annotation Click to see Annotation Detail View

References

References - curated

#	Reference Title	Reference Citation
1.	Functional primordial germ cell-like cells from pluripotent stem cells in rats.	Oikawa M, etal., Science. 2022 Apr 8;376(6589):176-179. doi: 10.1126/science.abl4412. Epub 2022 Apr 7.
2.	Strains registration through online submission tool	Strains registered through the Online Strain Submission Tool

Region

Additional Information

Gene Annotator (Functional Annotation) unavailable	Gene Annotator (Annotation Distribution) unavailable	Variant Visualizer (Genomic Variants) unavailble Variant Visualizer (Genomic Variants) unavailable
Gene Annotator (Functional Annotation)	Gene Annotator (Annotation Distribution)	Variant Visualizer (Genomic Variants)

InterViewer (Protein-Protein Interactions) unavailable	Gviewer (Genome Viewer) unavailable	Variant Visualizer (Damaging Variants) unavailble Variant Visualizer (Damaging Variants) unavailable
InterViewer (Protein-Protein Interactions)	GViewer (Genome Viewer)	Variant Visualizer (Damaging Variants)

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MOET (Multi-Ontology Enrichement)	GOLF (Gene-Ortholog Location Finder)

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Strain: WI-Nanos3em1(tdTomato)Nips

Strain: WI-Nanos3^{em1(tdTomato)Nips}