Cyagen Biosciences (Guangzhou) Inc.Building D, 3rd Floor, 3 Juquan Road, Science City, Guangzhou, 510663, China
Description:
The gRNA to rat Mertk gene, and Cas9 mRNA were co-injected into fertilized rat eggs to generate targeted knockout offspring. F0 founder animals were identified by PCR followed by sequence analysis, which were bred to wildtype rat to test germline transmission and F1 animal generation. This strain was derived from founder 6# carrying a 13934 deletion which included exons 3 to 5.