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Strain Registration

Strain: WI;WDB-ROSA26tm1(H2B-tdTomato)/Nips

Symbol: WI;WDB-ROSA26tm1(H2B-tdTomato)/Nips
Strain: WI;WDB-ROSA26tm1(H2B-tdTomato)
Substrain: Nips
RGD ID: 155269039
Citation ID: RRID:RGD_155269039
Ontology ID: RS:0005168
Previously known as: WI.WDB-ROSA26tm1(H2B-tdTomato)/Nips; WI.WDB-ROSA26tm1(H2BC11-tdTomato)/Nips
Type: mutant
Source: Section of Mammalian Transgenesis Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki Aichi, JAPAN
Origin: PCR products of the human H2BC11 (H2B-6), tdTomato, splice acceptor sequence, and IRES-Neor-SV40pA were inserted into the NheI site of prROSA26-1 with an in-fusion cloning kit. The final tdTomato-H2B-6 targeting vector was linearized by SalI digestion. The vector was introduced into WDB/Nips-ES1/Nips (RGD ID:10054010) embryonic stem cells by electroporation. Targeted ES cells were injected into Crlj:WI (RGD ID: 2312504) blastocysts to produce chimeric rats. The chimeric rats were crossed with Crlj:WI rats to produce heterozygous founder rats.These rat strains are being maintained by crossing the founder rats with Crlj:WI rats. ROSA26 is a synonym for rat Thumpd3-as1 (RGD:6491660) and is used as an official symbol for rat strain nomenclature.
Genetic Status: Heterozygous
Last Known Status: Cryopreserved Embryo (as of 2022-10-11)






References

References - curated
# Reference Title Reference Citation
1. Strains registration through online submission tool Strains registered through the Online Strain Submission Tool

Region


Additional Information


Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2023-06-06 WI;WDB-ROSA26tm1(H2B-tdTomato)/Nips    WI.WDB-ROSA26tm1(H2B-tdTomato)/Nips    Symbol and/or name change 68913 APPROVED
2022-10-13 WI.WDB-ROSA26tm1(H2B-tdTomato)/Nips    WI.WDB-ROSA26tm1(H2BC11-tdTomato)/Nips    Symbol and/or name change 68913 APPROVED
2022-10-12 WI.WDB-ROSA26tm1(H2BC11-tdTomato)/Nips    WI;WDB-ROSA26tm1(H2BC11-tdTomato)/Nips    Symbol and/or name change 68913 APPROVED
2022-10-11 WI;WDB-ROSA26tm1(H2BC11-tdTomato)/Nips    WI;WDB-ROSA26tm1(H2B-tdTomato)/Nips    Symbol and/or name change 68913 APPROVED