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Strain Registration

Strain: SD-Tg(Ddx4-Cas9)SynblRrrc

Symbol: SD-Tg(Ddx4-Cas9)SynblRrrc
Strain: SD-Tg(Ddx4-Cas9)
Substrain: SynblRrrc
RGD ID: 155260362
Citation ID: RRID:RRRC_00969
Ontology ID: RS:0005161
Also Known As: SD-Tg(Ddx4-Cas9)Rrrc; RRRC:00969; Ddx4-Cas9
Type: transgenic
Available Source: Rat Resource and Research Center
Origination: Rat Resource and Research Center
Description: rat strain designed to express Cas9 under the control of the rat Ddx4 (Vasa) regulatory elements while also retaining expression of Ddx4. The strategy entailed insertion of a P2A sequence followed by human-codon-optimized Cas9 coding sequences with nuclear localization signals near the C-terminus of the rat Ddx4 (Vasa) gene. Animals express Cas9 in the testis in the spermatogonial layer and in meiosis stages with 4N DNA content. Strain catalyzes high levels of Active Genetic Gene conversion when paired with certain Active Genetic cassettes. ROBUST AND EFFICIENT ACTIVE GENETICS GENE CONVERSION IN THE RAT AND MOUSE Chenyen Lai, Oscar Alvarez, Kristen Read, Don van Fossan,Christopher M Conner, Shannon Xaing-Ru Xu, Dale O. Cowley, Valentino Gantz, David R. Webb, Kurt Jarnagin URL: Doi:10.1101/2022.08.30.505951 URL: Doi:10.1101/2022.08.30.505951
Genetic Status: Heterozygous
Last Known Status: Unknown
Research Usage Genetics, Reproductive Biology, Gene Conversion, Active Genetics






References

References - curated
# Reference Title Reference Citation
1. Strains registration through online submission tool Strains registered through the Online Strain Submission Tool

Region


Additional Information


Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2022-10-06 SD-Tg(Ddx4-Cas9)SynblRrrc    SD-Tg(Ddx4-Cas9)Rrrc    Symbol and/or name change 68913 APPROVED

Database Acc Id Source(s)
RRRC RRRC:00969 RGD