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Strain Registration

Strain: SD-Tg(Sycp1-Cas9-eGFP)SynblRrrc

Symbol: SD-Tg(Sycp1-Cas9-eGFP)SynblRrrc
Strain: SD-Tg(Sycp1-Cas9-eGFP)
Substrain: SynblRrrc
RGD ID: 155260361
Citation ID: RRID:RRRC_00968
Ontology ID: RS:0005159
Also Known As: SD-Tg(Sycp1-Cas9-eGFP)Rrrc; RRRC:00968; Sycp1-Cas9-T2A-eGFP
Type: transgenic
Available Source: Rat Resource and Research Center
Origination: Rat Resource and Research Center
Description: This project entailed generation of a novel rat strain in which a P2A-Cas9-T2A-EGFP coding sequence was inserted at the stop codon of the rat Sycp1 gene for co-expression of Cas9 and EGFP under the control of the Sycp1 regulatory elements. Animals express Cas9 in the testis in the spermatogonial layer and in meiosis stages with 4N DNA content. Strain catalyzes high levels of Active Genetic Gene conversion when paired with certain Active Genetic cassettes. ROBUST AND EFFICIENT ACTIVE GENETICS GENE CONVERSION IN THE RAT AND MOUSE Chenyen Lai, Oscar Alvarez, Kristen Read, Don van Fossan,Christopher M Conner, Shannon Xaing-Ru Xu, Dale O. Cowley, Valentino Gantz, David R. Webb, Kurt Jarnagin URL: Doi:10.1101/2022.08.30.505951
Genetic Status: Heterozygous
Last Known Status: Cryopreserved Sperm (as of 2022-10-06)
Research Usage Genetics, Reproductive Biology, Gene Conversion, Active Genetics






References

References - curated
# Reference Title Reference Citation
1. Strains registration through online submission tool Strains registered through the Online Strain Submission Tool

Region


Additional Information


Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2022-10-06 SD-Tg(Sycp1-Cas9-eGFP)SynblRrrc    SD-Tg(Sycp1-Cas9-eGFP)Rrrc    Symbol and/or name change 68913 APPROVED

Database Acc Id Source(s)
RRRC RRRC:00968 RGD