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Strain Registration

Strain: LE-ROSA26em1(CAG-tdTomato)Rrrc

Symbol: LE-ROSA26em1(CAG-tdTomato)Rrrc
Strain: LE-ROSA26em1(CAG-tdTomato)
Substrain: Rrrc
RGD ID: 150520182
Citation ID: RRID:RRRC_00938
Ontology ID: RS:0005192
Also Known As: LE-Rosa26 (CAG-LSL-TdTomato)em1Rrrc; LE-ROSA26^[em1(CAG-tdTomato)Rrrc]
Type: mutant
Available Source: Rat Resource and Research Center
Origination: Rat Resource and Research Center
Description: The DNA construct was made by replacing ZsGreen with TdTomato in plasmid pCAG-loxP-STOP-loxP-ZsGreen (Addgene plasmid #51269, donated by Pawel Pelczar). CRISPR/Cas9 genome editing technology was used to knock in the DNA construct into the rat ROSA26 locus in the embryos from outbred BluHsd:LE. Reporter line that can be crossed with Cre-expressing strains. In presence of Cre, the floxed "STOP" upstream of the TdTomato gene is deleted allowing expression of the red fluorescent TdTomato protein. ROSA26 is a synonym for rat Thumpd3-as1 (RGD:6491660) and is used as an official symbol for rat strain nomenclature.
Last Known Status: Live Animals (as of 2023-08-01)






References

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Additional Information

Database Acc Id Source(s)
RRRC RRRC:00938 RGD