Strain Report - Rat Genome Database

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Strain: SS-Kcnj1em1Kasu+/-

Symbol: SS-Kcnj1em1Kasu+/-
Strain: SS-Kcnj1em1+/Kcnj1Kcnj1em1-
Substrain: Kasu
RGD ID: 13782351
Citation ID: RRID:RGD_13782351
Ontology ID: RS:0004613
Alleles: Kcnj1em1Kasu
Previously known as: ROMK+/-
Type: mutant
Source: Departments of Cardiovascular Diseases, Merck Research Laboratories, Merck&Co.,Inc
Origin: Zinc Finger Nuclease (ZFN) was utilized to knockout rat Kcnj1 function. ZFN constructs targeting the gene were designed and purchased from Sigma Aldrich (St. Louis, MO, USA). The targeting site sequence is TCAAGTGACCATAGGTTACGgattcaGGTTTGTGAC (lower case represents the ZFN cleavage site). Kcnj1 knockout founders were generated by microinjection of ZFN mRNAs into single -cell SS (from Harlan) rat embryos. The resulting mutation is 209 bp deletion from G225 to G433, leading to frameshift and premature termination of Kcnj1 protein in homozygotes.
Genetic Status: Heterozygous
Last Known Status: Unknown
Position
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.2830,779,883 - 30,808,607RGD_MAPPER_PIPELINEmRatBN7.2
Rnor_6.0833,490,280 - 33,519,127RGD_MAPPER_PIPELINERnor6.0
Rnor_5.0833,453,597 - 33,454,750RGD_MAPPER_PIPELINERnor5.0
RGSC_v3.4832,158,243 - 32,162,370RGD_MAPPER_PIPELINERGSC3.4





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Mammalian Phenotype

References

References - curated
# Reference Title Reference Citation
1. Heterozygous disruption of renal outer medullary potassium channel in rats is associated with reduced blood pressure. Zhou X, etal., Hypertension. 2013 Aug;62(2):288-94. doi: 10.1161/HYPERTENSIONAHA.111.01051. Epub 2013 Jun 10.

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