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Strain: SS-Kcnj1em1Kasu+/+

Symbol: SS-Kcnj1em1Kasu+/+
Strain: SS-Kcnj1em1+/Kcnj1Kcnj1em1+
Substrain: Kasu
RGD ID: 13782280
Citation ID: RRID:RGD_13782280
Ontology ID: RS:0004609
Previously known as: ROMK+/+
Type: mutant
Source: Departments of Cardiovascular Diseases, Merck Research Laboratories, Merck&Co.,Inc
Origin: Zinc Finger Nuclease (ZFN) was utilized to knockout rat Kcnj1 function. ZFN constructs targeting the gene were designed and purchased from Sigma Aldrich (St. Louis, MO, USA). The targeting site sequence is CTCAAGTGACCATAGGTTACGgattcaGGTTTGTGAC (lower case represents the ZFN cleavage site). Kcnj1 knockout founders were generated by microinjection of ZFN mRNAs into single-cell SS (from Harlan) rat embryos. The resulting mutation is 209 bp deletion from G225 to G433, leading to frameshift and premature termination of Kcnj1 protein. This strain is the wild type littermate from heterozygous cross.
Genetic Status: Wild Type
Last Known Status: Unknown


References - curated
# Reference Title Reference Citation
1. Heterozygous disruption of renal outer medullary potassium channel in rats is associated with reduced blood pressure. Zhou X, etal., Hypertension. 2013 Aug;62(2):288-94. doi: 10.1161/HYPERTENSIONAHA.111.01051. Epub 2013 Jun 10.


Additional Information

Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2018-09-06 SS-Kcnj1em1Kasu+/+    SS-Kcnj1em1Kasu    Symbol updated 68687 APPROVED
2018-09-06 SS-Kcnj1em1Kasu+/+    SS-Kcnj1em1Kasu    Symbol updated 68687 APPROVED