Department of Experimental Pathology, Cancer Institute, Toshima-ku, Tokyo, Japan
Description:
Wild type Tsc2 transgene was constructed from the Tsc2 cDNA from BN rat and was microinjected into single male pronuclei. Eggs were cultured and tranferred into female wistar which were mated with Eker rats.
N-ethyl-N-nitrosourea (ENU) was used to induce lesion induction, expression of the wild type Tsc2 transgene is associated with a suppression of the N-ethyl-N-nitrosourea-induced renal carcinogenesis detected in non-transgenic Eker/+ heterozygotes
This has a DNA fragment 710 bp which covers 5UR to exon 2 this lacks the alternative splicing exons and the SV40 poly(A) sequence. This has 3 copies of the transgene.