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Strain: Eker-Tg(Tsc2)28Hin

Symbol: Eker-Tg(Tsc2)28Hin
Strain: Eker-Tg(Tsc2)28
Substrain: Hin
RGD ID: 1357182
Citation ID: RRID:RGD_1357182
Ontology ID: RS:0000265
Alleles: Tsc2
Type: transgenic
Available Source: Not Available
Origination: Department of Experimental Pathology, Cancer Institute, Toshima-ku, Tokyo, Japan
Description: Wild type Tsc2 transgene was constructed from the Tsc2 cDNA from BN rat and was microinjected into single male pronuclei. Eggs were cultured and tranferred into female wistar which were mated with Eker rats.
Last Known Status: Unknown






References

References - curated
# Reference Title Reference Citation
1. Transgenic rescue from embryonic lethality and renal carcinogenesis in the Eker rat model by introduction of a wild-type Tsc2 gene. Kobayashi T, etal., Proc Natl Acad Sci U S A 1997 Apr 15;94(8):3990-3.
2. RGD Strain RSO annotation pipeline RGD Automated Pipelines

Region


Additional Information

RGD Curation Notes
Note Type Note Reference
strain_drgs_chems N-ethyl-N-nitrosourea (ENU) was used to induce lesion induction, expression of the wild type Tsc2 transgene is associated with a suppression of the N-ethyl-N-nitrosourea-induced renal carcinogenesis detected in non-transgenic Eker/+ heterozygotes 628401
strain_life_disease expression of the wild type Tsc2 transgene rescues Tsc2Eker homozygotes from the otherwise expected embryonic lethality in the Eker strain 628401
strain_phys_biochem This has a DNA fragment 710 bp which covers 5UR to exon 2 this lacks the alternative splicing exons and the SV40 poly(A) sequence. This has 3 copies of the transgene. 628401
strain_reproduction When these were mated with Eker carriers the offsprings were Tsc2 homozygous. 628401