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Strain: F344-Nppcem4Kyo

Symbol: F344-Nppcem4Kyo
Strain: F344-Nppcem4
Substrain: Kyo
RGD ID: 127284872
Citation ID: RRID:RGD_127284872
Ontology ID: RS:0004955
Alleles: Nppcem4Kyo
Previously known as: delta 774 mutant
Type: mutant
Source: National BioResource Project for the Rat in Japan
Origin: This strain was established by injecting Zinc-finger nucleases (ZFNs) to F344/Stm pronuclear stage embryos. Selected ZFNs were designed to target exon 1 of rat Nppc, which encodes the N-terminus of natriuretic peptide precursor C (target sequence: CGAAGCCAAGCCCGGGACaccaccGAAGGTGGGTGCTGTCGCG; nucleotides 179 - 221, NC_005108.4). The injected F344/Stm embryos were transferred into the oviducts of pseudopregnant rats. Four lines of knockout strains were estaglished. This F344-Nppcem4Kyo strain ( delta 774) had a massive deletion within the Nppc gene that included the translation initiation site. Quantitative RT-PCR revealed that the expression of Nppc mRNA in the brain was drastically decreased in homozygous delta 774 mutant rats.
Genetic Status: Homozygous
Coat Color: Albino
Last Known Status: Unknown
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.2987,320,051 - 87,324,251RGD_MAPPER_PIPELINEmRatBN7.2
Rnor_6.0993,731,436 - 93,735,636RGD_MAPPER_PIPELINERnor6.0
Rnor_5.0993,458,753 - 93,462,953RGD_MAPPER_PIPELINERnor5.0
RGSC_v3.4985,434,254 - 85,438,454RGD_MAPPER_PIPELINERGSC3.4

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Mammalian Phenotype


References - curated
# Reference Title Reference Citation
1. Rats deficient C-type natriuretic peptide suffer from impaired skeletal growth without early death. Fujii T, etal., PLoS One. 2018 Mar 22;13(3):e0194812. doi: 10.1371/journal.pone.0194812. eCollection 2018.


Additional Information