Strain Report - Rat Genome Database

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Strain: F344-Nppcem2Kyo

Symbol: F344-Nppcem2Kyo
Strain: F344-Nppcem2
Substrain: Kyo
RGD ID: 127284868
Citation ID: RRID:RGD_127284868
Ontology ID: RS:0004953
Alleles: Nppcem2Kyo
Previously known as: delta 9 mutant
Type: mutant
Source: National BioResource Project for the Rat in Japan
Origin: This strain was established by injecting Zinc-finger nucleases (ZFNs) to F344/Stm pronuclear stage embryos. Selected ZFNs were designed to target exon 1 of rat Nppc, which encodes the N-terminus of natriuretic peptide precursor C (target sequence: CGAAGCCAAGCCCGGGACaccaccGAAGGTGGGTGCTGTCGCG; nucleotides 179 - 221, NC_005108.4). The injected F344/Stm embryos were transferred into the oviducts of pseudopregnant rats. Four lines of knockout strains were estaglished. This F344-Nppcem2Kyo strain ( delta 9) was deduced to generate one amino-acid substitution (a.a. 26, from Gly to Ala, NP_446202, at nucleotides 192 - 194, NM_053750.1) and a three amino-acid deletion (a.a. 27 - 29, Thr, Pro and Pro, NP_446202, at nucleotides 193 - 201, NM_053750.1), within the N-terminal portion of the full-length Nppc
Coat Color: Albino
Last Known Status: Unknown
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.2987,320,051 - 87,324,251RGD_MAPPER_PIPELINEmRatBN7.2
Rnor_6.0993,731,436 - 93,735,636RGD_MAPPER_PIPELINERnor6.0
Rnor_5.0993,458,753 - 93,462,953RGD_MAPPER_PIPELINERnor5.0
RGSC_v3.4985,434,254 - 85,438,454RGD_MAPPER_PIPELINERGSC3.4


References - curated
# Reference Title Reference Citation
1. Rats deficient C-type natriuretic peptide suffer from impaired skeletal growth without early death. Fujii T, etal., PLoS One. 2018 Mar 22;13(3):e0194812. doi: 10.1371/journal.pone.0194812. eCollection 2018.


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