Strain Report - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Strain: SD-Cftrem2Ang

Symbol: SD-Cftrem2Ang
Strain: SD-Cftrem2
Substrain: Ang
RGD ID: 126925994
Citation ID: RRID:RGD_126925994
Ontology ID: RS:0005022
Alleles: Cftrem2Ang
Also Known As: CFTR KO rats; SD-Cftr^[em2Ang]
Type: mutant
Available Source: Not Available
Origination: INSERM 1151 INEM Universit? de Paris Paris France.
Description: The CRISPR/Cas9 system targeting at exon 3 to create gene knock out was injected to the male pronucleus of the fertalized one-cell stage embryos collected from Crl:SD. The donor DNA generated a frameshift and the creation of one XbaI restriction site and a premature stop codon. Two knockout founders ( referred as MUKORAT8.3 and MUKORAT 6.4) exhibit no difference in phenotypes and were pooled for study.
Genetic Status: Homozygous
Last Known Status: Unknown
Position
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.2446,561,269 - 46,728,759RGD_MAPPER_PIPELINEmRatBN7.2
Rnor_6.0442,693,263 - 42,860,679RGD_MAPPER_PIPELINERnor6.0
Rnor_5.0442,281,040 - 42,448,571RGD_MAPPER_PIPELINERnor5.0
RGSC_v3.4443,874,852 - 44,041,870RGD_MAPPER_PIPELINERGSC3.4





References

References - curated
# Reference Title Reference Citation
1. Characterization of two rat models of cystic fibrosis-KO and F508del CFTR-Generated by Crispr-Cas9. Dreano E, etal., Animal Model Exp Med. 2019 Nov 25;2(4):297-311. doi: 10.1002/ame2.12091. eCollection 2019 Dec.

Region


Additional Information