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Strain: SD-Ube3aem1Jue

Symbol: SD-Ube3aem1Jue
Strain: SD-Ube3aem1
Substrain: Jue
RGD ID: 126790464
Citation ID: RRID:RGD_126790464
Ontology ID: RS:0004921
Alleles: Ube3aem1Jue
Also Known As: SD-Ube3a^[em1Jue]
Type: mutant
Available Source: Not Available
Description: The CRISPR/Cas9 genome editing system was injected into fertilized Sprague-Dawley rat embryos and inserted into a surrogate. Two genomic RNAs (gRNAs) were designed to target the 5ʹ-end of the Ube3a gene (upstream of the Ube3a coding sequence) and two gRNAs target sequences downstream of Ube3a. gRNA pairs were used on each end of the deletion to maximize the probability of a complete deletion of the 90-kb region encompassing the Ube3a gene. The 90 kb deletion in Ube3a was confirmed by genome sequencing. Only pups with maternal inheritance of the deletion carried traits for model of Angleman Syndrome.
Last Known Status: Unknown
Position
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.21110,070,260 - 110,161,675RGD_MAPPER_PIPELINEmRatBN7.2
Rnor_6.01116,586,901 - 116,678,161RGD_MAPPER_PIPELINERnor6.0
Rnor_5.01117,745,283 - 117,834,011RGD_MAPPER_PIPELINERnor5.0
RGSC_v3.41110,729,142 - 110,816,491RGD_MAPPER_PIPELINERGSC3.4





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References

References - curated
# Reference Title Reference Citation
1. Translational outcomes in a full gene deletion of ubiquitin protein ligase E3A rat model of Angelman syndrome. Berg EL, etal., Transl Psychiatry. 2020 Jan 27;10(1):39. doi: 10.1038/s41398-020-0720-2.

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