RGD Reference Report - Gene expression profiles of cardiomyocytes in rat autoimmune myocarditis by DNA microarray and increase of regenerating gene family. - Rat Genome Database

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Gene expression profiles of cardiomyocytes in rat autoimmune myocarditis by DNA microarray and increase of regenerating gene family.

Authors: Watanabe, R  Hanawa, H  Yoshida, T  Ito, M  Isoda, M  Chang, H  Toba, K  Yoshida, K  Kojima, M  Otaki, K  Ding, L  Hao, K  Kato, K  Kodama, M  Aizawa, Y 
Citation: Watanabe R, etal., Transl Res. 2008 Sep;152(3):119-27. doi: 10.1016/j.trsl.2008.07.006. Epub 2008 Aug 20.
RGD ID: 9831381
Pubmed: PMID:18774541   (View Abstract at PubMed)
DOI: DOI:10.1016/j.trsl.2008.07.006   (Journal Full-text)

Cardiomyocytes with myocarditis compared with the normal state are thought to change the expressions of various genes greatly, some of which may be new biomarkers or new biologic medicinal products. However, until now, little comprehensive analysis has been made of gene-expression changes in cardiomyocytes with myocarditis. In this study, we performed a DNA microarray analysis by using cardiomyocytes from rat experimental autoimmune myocarditis (EAM). On day 0, rats were immunized with porcine cardiac myosin and cardiomyocytes were isolated and purified from EAM hearts and normal hearts by a method that is hardly thought to change gene expressions in cardiomyocytes. RNA from normal cardiomyocytes and cardiomyocytes of EAM on day 18 was analyzed for 7711 gene expressions by DNA microarray. Some gene expressions showed over 10-fold changes. In particular, the regenerated gene (Reg)2/pancreatitis-associated protein (PAP)1 messenger RNA (mRNA) level most markedly increased in the genes, which were clearly expressed in cardiomyocytes rather than in noncardiomyocytes, and it was approximately 2000-fold greater in cardiomyocytes under active myocarditis than normal by real-time reverse transcription polymerase chain reaction analysis. Moreover, we demonstrated that Reg2/PAP1 proteins determined by Western blot analysis and immunohistochemistry and other Reg/PAP family gene expressions were remarkably increased in EAM hearts; in addition, interleukin (IL)-6 expression was significantly related to Reg2/PAP1. It seemed that these data were useful as a reference database of gene-expression changes in cardiomyocytes with myocarditis. The Reg/PAP family, which was found to show dramatically increasing gene expressions by DNA microarray analysis, was suspected to play an important role in myocarditis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Autoimmune Myocarditis  ISOReg3b (Rattus norvegicus)9831381; 9831381mRNA:increased expression:heart and cardiomyocyte (rat)RGD 
Experimental Autoimmune Myocarditis  IEP 9831381mRNA:increased expression:heart and cardiomyocyte (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Reg3b  (regenerating family member 3 beta)

Genes (Mus musculus)
Reg3b  (regenerating islet-derived 3 beta)

Genes (Homo sapiens)
REG3A  (regenerating family member 3 alpha)


Additional Information