RGD Reference Report - The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein. - Rat Genome Database

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The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein.

Authors: Lin, P  Le-Niculescu, H  Hofmeister, R  McCaffery, JM  Jin, M  Hennemann, H  McQuistan, T  De Vries, L  Farquhar, MG 
Citation: Lin P, etal., J Cell Biol. 1998 Jun 29;141(7):1515-27.
RGD ID: 9831131
Pubmed: PMID:9647645   (View Abstract at PubMed)
PMCID: PMC2132997   (View Article at PubMed Central)

We have identified CALNUC, an EF-hand, Ca2+-binding protein, as a Golgi resident protein. CALNUC corresponds to a previously identified EF-hand/calcium-binding protein known as nucleobindin. CALNUC interacts with Galphai3 subunits in the yeast two-hybrid system and in GST-CALNUC pull-down assays. Analysis of deletion mutants demonstrated that the EF-hand and intervening acidic regions are the site of CALNUC's interaction with Galphai3. CALNUC is found in both cytosolic and membrane fractions. The membrane pool is tightly associated with the luminal surface of Golgi membranes. CALNUC is widely expressed, as it is detected by immunofluorescence in the Golgi region of all tissues and cell lines examined. By immunoelectron microscopy, CALNUC is localized to cis-Golgi cisternae and the cis-Golgi network (CGN). CALNUC is the major Ca2+-binding protein detected by 45Ca2+-binding assay on Golgi fractions. The properties of CALNUC and its high homology to calreticulin suggest that it may play a key role in calcium homeostasis in the CGN and cis-Golgi cisternae.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nucb1Ratextrinsic component of Golgi membrane  IDA  RGD 
Nucb1RatGolgi cisterna membrane  IDA  RGD 
Nucb1RatGolgi-associated vesicle  IDA  RGD 
Nucb1Ratlumenal side of Golgi membrane  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nucb1Ratcalcium ion binding  IDA  RGD 
Nucb1RatG-protein alpha-subunit binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nucb1  (nucleobindin 1)


Additional Information