RGD Reference Report - Comparative gene expression profile of mouse carotid body and adrenal medulla under physiological hypoxia. - Rat Genome Database

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Comparative gene expression profile of mouse carotid body and adrenal medulla under physiological hypoxia.

Authors: Ganfornina, MD  Perez-Garcia, MT  Gutierrez, G  Miguel-Velado, E  Lopez-Lopez, JR  Marin, A  Sanchez, D  Gonzalez, C 
Citation: Ganfornina MD, etal., J Physiol. 2005 Jul 15;566(Pt 2):491-503. Epub 2005 May 12.
RGD ID: 9743958
Pubmed: PMID:15890701   (View Abstract at PubMed)
PMCID: PMC1464746   (View Article at PubMed Central)
DOI: DOI:10.1113/jphysiol.2005.088815   (Journal Full-text)

The carotid body (CB) is an arterial chemoreceptor, bearing specialized type I cells that respond to hypoxia by closing specific K+ channels and releasing neurotransmitters to activate sensory axons. Despite having detailed information on the electrical and neurochemical changes triggered by hypoxia in CB, the knowledge of the molecular components involved in the signalling cascade of the hypoxic response is fragmentary. This study analyses the mouse CB transcriptional changes in response to low PO2 by hybridization to oligonucleotide microarrays. The transcripts were obtained from whole CBs after mice were exposed to either normoxia (21% O2), or physiological hypoxia (10% O2) for 24 h. The CB transcriptional profiles obtained under these environmental conditions were subtracted from the profile of control non-chemoreceptor adrenal medulla extracted from the same animals. Given the common developmental origin of these two organs, they share many properties but differ specifically in their response to O2. Our analysis revealed 751 probe sets regulated specifically in CB under hypoxia (388 up-regulated and 363 down-regulated). These results were corroborated by assessing the transcriptional changes of selected genes under physiological hypoxia with quantitative RT-PCR. Our microarray experiments revealed a number of CB-expressed genes (e.g. TH, ferritin and triosephosphate isomerase) that were known to change their expression under hypoxia. However, we also found novel genes that consistently changed their expression under physiological hypoxia. Among them, a group of ion channels show specific regulation in CB: the potassium channels Kir6.1 and Kcnn4 are up-regulated, while the modulatory subunit Kcnab1 is down-regulated by low PO2 levels.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
KCNAB1HumanHypoxia  ISOKcnab1 (Mus musculus)mRNA:decreased expression:carotid body (mouse)RGD 
Kcnab1RatHypoxia  ISOKcnab1 (Mus musculus)mRNA:decreased expression:carotid body (mouse)RGD 
Kcnab1MouseHypoxia  IEP mRNA:decreased expression:carotid body (mouse)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kcnab1  (potassium voltage-gated channel subfamily A regulatory beta subunit 1)

Genes (Mus musculus)
Kcnab1  (potassium voltage-gated channel, shaker-related subfamily, beta member 1)

Genes (Homo sapiens)
KCNAB1  (potassium voltage-gated channel subfamily A regulatory beta subunit 1)


Additional Information