RGD Reference Report - Negative elongation factor NELF controls transcription of immediate early genes in a stimulus-specific manner. - Rat Genome Database

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Negative elongation factor NELF controls transcription of immediate early genes in a stimulus-specific manner.

Authors: Fujita, T  Piuz, I  Schlegel, W 
Citation: Fujita T, etal., Exp Cell Res. 2009 Jan 15;315(2):274-84. doi: 10.1016/j.yexcr.2008.10.032. Epub 2008 Nov 5.
RGD ID: 9693721
Pubmed: PMID:19014935   (View Abstract at PubMed)
DOI: DOI:10.1016/j.yexcr.2008.10.032   (Journal Full-text)

The transcription rate of immediate early genes (IEGs) is controlled directly by transcription elongation factors at the transcription elongation step. Negative elongation factor (NELF) and 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) sensitivity-inducing factor (DSIF) stall RNA polymerase II (pol II) soon after transcription initiation. Upon induction of IEG transcription, DSIF is converted into an accelerator for pol II elongation. To address whether and how NELF as well as DSIF controls overall IEG transcription, its expression was reduced using stable RNA interference in GH4C1 cells. NELF knock-down reduced thyrotropin-releasing hormone (TRH)-induced transcription of the IEGs c-fos, MKP-1, and junB. In contrast, epidermal growth factor (EGF)-induced transcription of these IEGs was unaltered or even slightly increased by NELF knock-down. Thus, stable knock-down of NELF affects IEG transcription stimulation-specifically. Conversely, DSIF knock-down reduced both TRH- and EGF-induced transcription of the three IEGs. Interestingly, TRH-induced activation of the MAP kinase pathway, a pathway essential for transcription of the three IEGs, was down-regulated by NELF knock-down. Thus, stable knock-down of NELF, by modulating intracellular signaling pathways, caused stimulation-specific loss of IEG transcription. These observations indicate that NELF controls overall IEG transcription via multiple mechanisms both directly and indirectly.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NelfeRatpositive regulation of ERK1 and ERK2 cascade  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nelfe  (negative elongation factor complex member E)


Additional Information