RGD Reference Report - Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. - Rat Genome Database

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Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II.

Authors: Saha, RN  Wissink, EM  Bailey, ER  Zhao, M  Fargo, DC  Hwang, JY  Daigle, KR  Fenn, JD  Adelman, K  Dudek, SM 
Citation: Saha RN, etal., Nat Neurosci. 2011 May 29;14(7):848-56. doi: 10.1038/nn.2839.
RGD ID: 9693718
Pubmed: PMID:21623364   (View Abstract at PubMed)
PMCID: PMC3125443   (View Article at PubMed Central)
DOI: DOI:10.1038/nn.2839   (Journal Full-text)

Transcription of immediate early genes (IEGs) in neurons is highly sensitive to neuronal activity, but the mechanism underlying these early transcription events is largely unknown. We found that several IEGs, such as Arc (also known as Arg3.1), are poised for near-instantaneous transcription by the stalling of RNA polymerase II (Pol II) just downstream of the transcription start site in rat neurons. Depletion through RNA interference of negative elongation factor, a mediator of Pol II stalling, reduced the Pol II occupancy of the Arc promoter and compromised the rapid induction of Arc and other IEGs. In contrast, reduction of Pol II stalling did not prevent transcription of IEGs that were expressed later and largely lacked promoter-proximal Pol II stalling. Together, our data strongly indicate that the rapid induction of neuronal IEGs requires poised Pol II and suggest a role for this mechanism in a wide variety of transcription-dependent processes, including learning and memory.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NelfaRatpositive regulation of protein modification process  IMP histone H3-K4 tri-methylationRGD 
NelfeRatpositive regulation of protein modification process  IMP histone H3-K4 tri-methylationRGD 
NelfaRatpositive regulation of transcription by RNA polymerase II  IMP  RGD 
NelfeRatpositive regulation of transcription by RNA polymerase II  IMP  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NelfaRatchromatin binding  IMP  RGD 
NelfeRatchromatin binding  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nelfa  (negative elongation factor complex member A)
Nelfe  (negative elongation factor complex member E)


Additional Information