RGD Reference Report - Differential distribution of dynamin isoforms in mammalian cells. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Differential distribution of dynamin isoforms in mammalian cells.

Authors: Cao, H  Garcia, F  McNiven, MA 
Citation: Cao H, etal., Mol Biol Cell. 1998 Sep;9(9):2595-609.
RGD ID: 9685132
Pubmed: PMID:9725914   (View Abstract at PubMed)
PMCID: PMC25532   (View Article at PubMed Central)

Dynamins are 100-kDa GTPases that are essential for clathrin-coated vesicle formation during receptor-mediated endocytosis. To date, three different dynamin genes have been identified, with each gene expressing at least four different alternatively spliced forms. Currently, it is unclear whether these different dynamin gene products perform distinct or redundant cellular functions. Therefore, the focus of this study was to identify additional spliced variants of dynamin from rat tissues and to define the distribution of the dynamin family members in a cultured rat epithelial cell model (Clone 9 cells). After long-distance reverse transcription (RT)-PCR of mRNA from different rat tissues, the full-length cDNAs encoding the different dynamin isoforms were sequenced and revealed four additional spliced variants for dynamin I and nine for dynamin III. Thus, in rat tissues there are a total of at least 25 different mRNAs produced from the three dynamin genes. Subsequently, we generated stably transfected Clone 9 cells expressing full-length cDNAs of six different spliced forms tagged with green fluorescent protein. Confocal or fluorescence microscopy of these transfected cells revealed that many of the dynamin proteins associate with distinct membrane compartments, which include clathrin-coated pits at the plasma membrane and the Golgi apparatus, and several undefined vesicle populations. These results indicate that the dynamin family is more extensive than was originally predicted and suggest that the different dynamin proteins are localized to distinct cytoplasmic or membrane compartments.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Dnm2Ratclathrin-coated pit  IDA  RGD 
Dnm1Ratclathrin-coated vesicle located_inIDA PMID:9725914UniProt 
Dnm1RatGolgi apparatus located_inIDA PMID:9725914UniProt 
Dnm2Ratlamellipodium  IDA  RGD 
Dnm2Ratruffle membrane  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dnm1  (dynamin 1)
Dnm2  (dynamin 2)

Objects referenced in this article
Gene Dnm3 dynamin 3 Rattus norvegicus

Additional Information