RGD Reference Report - Transcription elongation factors are involved in programming hormone production in pituitary neuroendocrine GH4C1 cells. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Transcription elongation factors are involved in programming hormone production in pituitary neuroendocrine GH4C1 cells.

Authors: Fujita, T  Piuz, I  Schlegel, W 
Citation: Fujita T, etal., Mol Cell Endocrinol. 2010 May 5;319(1-2):63-70. doi: 10.1016/j.mce.2010.01.020. Epub 2010 Jan 25.
RGD ID: 9588536
Pubmed: PMID:20097260   (View Abstract at PubMed)
DOI: DOI:10.1016/j.mce.2010.01.020   (Journal Full-text)

Transcription elongation of many eukaryotic genes is regulated. Two negative transcription elongation factors, 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) sensitivity-inducing factor (DSIF) and negative elongation factor (NELF) are known to stall collaboratively RNA polymerase II promoter proximally. We discovered that DSIF and NELF are linked to hormone expression in rat pituitary GH4C1 cells. When NELF-E, a subunit of NELF or Spt5, a subunit of DSIF was stably knocked-down, prolactin (PRL) expression was increased both at the mRNA and protein levels. In contrast, stable knock-down of only Spt5 abolished growth hormone (GH) expression. Transient NELF-E knock-down increased coincidentally PRL expression and enhanced transcription of a PRL-promoter reporter gene. However, no direct interaction of NELF with the PRL gene could be demonstrated by chromatin immuno-precipitation. Thus, NELF suppressed PRL promoter activity indirectly. In conclusion, transcription regulation by NELF and DSIF is continuously involved in the control of hormone production and may contribute to neuroendocrine cell differentiation.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NelfeRatnegative regulation of transcription by RNA polymerase II  IMP  RGD 
Supt5hRatpositive regulation of transcription by RNA polymerase II  IMP  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NelfeRatchromatin binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nelfe  (negative elongation factor complex member E)
Supt5h  (SPT5 homolog, DSIF elongation factor subunit)


Additional Information