RGD Reference Report - Assessment of androgen replacement therapy for erectile function in rats with type 2 diabetes mellitus by examining nitric oxide-related and inflammatory factors. - Rat Genome Database

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Assessment of androgen replacement therapy for erectile function in rats with type 2 diabetes mellitus by examining nitric oxide-related and inflammatory factors.

Authors: Kataoka, T  Hotta, Y  Maeda, Y  Kimura, K 
Citation: Kataoka T, etal., J Sex Med. 2014 Apr;11(4):920-9. doi: 10.1111/jsm.12447. Epub 2014 Jan 28.
RGD ID: 9495931
Pubmed: PMID:24467772   (View Abstract at PubMed)
DOI: DOI:10.1111/jsm.12447   (Journal Full-text)

INTRODUCTION: Type 2 diabetes mellitus (T2DM) has become a major public health issue and is considered a risk factor for erectile dysfunction (ED). T2DM is also associated with androgen deficiency. However, there have been few basic studies on androgen replacement therapy (ART) for ED treatment in T2DM animal models, and the mechanism underlying the effect of ART on T2DM-induced ED is unclear. AIM: To investigate the effect of ART on ED in T2DM rats by examining inflammatory and nitric oxide (NO)-related factors. METHODS: Otsuka Long-Evans Tokushima Fatty (OLETF) rats and their controls, Long-Evans Tokushima Otsuka (LETO) rats, were distributed into three groups: LETO, OLETF, and ART. In the ART group, OLETF rats were treated daily with testosterone (3 mg/kg/day, subcutaneously) from 20 to 25 weeks of age; LETO and OLETF rats received vehicle only. MAIN OUTCOME MEASURES: We measured erectile function by using measurements of the ratio between intracavernosal pressure (ICP) and mean arterial pressure (MAP) following electrical stimulation of the cavernous nerve and by evaluating the endothelial function of the corpus cavernosum in an isometric tension study. Expression of endothelial NO synthase (eNOS), inducible NO synthase (iNOS), sirtuin-1 (Sirt1), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-alpha) mRNA was detected using polymerase chain reaction. RESULTS: The ICP/MAP ratio in the OLETF group was significantly decreased and that in the ART group was significantly improved (P < 0.01). The response to acetylcholine was significantly decreased in the OLETF group and improved in the ART group (P < 0.01). Although expression of eNOS and Sirt1 mRNA was decreased and that of iNOS, IL-6, and TNF-alpha mRNA was increased in the OLETF group, ART improved mRNA expression. CONCLUSIONS: ART suppressed inflammation in rats with T2DM and metabolic disorders and improved their endothelial and erectile functions. ART could be effective for T2DM-induced ED and may be considered a potential ED treatment method.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IL6Humanimpotence treatmentISOIl6 (Rattus norvegicus)associated with Diabetes Mellitus and Type 2RGD 
Il6Ratimpotence treatmentIEP associated with Diabetes Mellitus and Type 2RGD 
Il6Mouseimpotence treatmentISOIl6 (Rattus norvegicus)associated with Diabetes Mellitus and Type 2RGD 
SIRT1Humanimpotence treatmentISOSirt1 (Rattus norvegicus)associated with Diabetes Mellitus and Type 2RGD 
Sirt1Ratimpotence treatmentIEP associated with Diabetes Mellitus and Type 2RGD 
Sirt1Mouseimpotence treatmentISOSirt1 (Rattus norvegicus)associated with Diabetes Mellitus and Type 2RGD 
TNFHumanimpotence treatmentISOTnf (Rattus norvegicus)associated with Diabetes Mellitus and Type 2RGD 
TnfRatimpotence treatmentIEP associated with Diabetes Mellitus and Type 2RGD 
TnfMouseimpotence treatmentISOTnf (Rattus norvegicus)associated with Diabetes Mellitus and Type 2RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il6  (interleukin 6)
Sirt1  (sirtuin 1)
Tnf  (tumor necrosis factor)

Genes (Mus musculus)
Il6  (interleukin 6)
Sirt1  (sirtuin 1)
Tnf  (tumor necrosis factor)

Genes (Homo sapiens)
IL6  (interleukin 6)
SIRT1  (sirtuin 1)
TNF  (tumor necrosis factor)


Additional Information