RGD Reference Report - The influence of sex on the chondrogenic potential of muscle-derived stem cells: implications for cartilage regeneration and repair. - Rat Genome Database

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The influence of sex on the chondrogenic potential of muscle-derived stem cells: implications for cartilage regeneration and repair.

Authors: Matsumoto, T  Kubo, S  Meszaros, LB  Corsi, KA  Cooper, GM  Li, G  Usas, A  Osawa, A  Fu, FH  Huard, J 
Citation: Matsumoto T, etal., Arthritis Rheum. 2008 Dec;58(12):3809-19. doi: 10.1002/art.24125.
RGD ID: 9068397
Pubmed: PMID:19035511   (View Abstract at PubMed)
DOI: DOI:10.1002/art.24125   (Journal Full-text)

OBJECTIVE: To explore possible differences in muscle-derived stem cell (MDSC) chondrogenic differentiation in vitro and articular cartilage regeneration in vivo between murine male MDSCs (M-MDSCs) and female MDSCs (F-MDSCs). METHODS: Three different populations of M- and F-MDSCs (n = 3 of each sex) obtained via preplate technique, which separates cells based on their variable adhesion characteristics, were compared for their in vitro chondrogenic potential using pellet culture. Cells were assayed with and without retroviral transduction to express bone morphogenetic protein 4 (BMP-4). The influence of both expression of stem cell marker Sca1 and in vitro expansion on the chondrogenic potential of M- and F-MDSCs was also determined. Additionally, BMP-4-transduced M- and F-MDSCs were applied to a full-thickness articular cartilage defect (n = 5 each) on the femur of a nude rat, and the quality of the repaired tissue was evaluated by macroscopic and histologic examination. RESULTS: With and without BMP-4 gene transduction, M-MDSCs produced significantly larger pellets with a richer extracellular matrix, compared with F-MDSCs. Sca1 purification influenced the chondrogenic potential of MDSCs, especially M-MDSCs. Long-term culture did not affect the chondrogenic potential of M-MDSCs but did influence F-MDSCs. M-MDSCs repaired articular cartilage defects more effectively than did F-MDSCs at all time points tested, as assessed both macroscopically and histologically. CONCLUSION: Our findings demonstrate that sex influences the chondrogenic differentiation and articular cartilage regeneration potential of MDSCs. Compared with female MDSCs, male MDSCs display more chondrogenic differentiation and better cartilage regeneration potential.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
BMP4HumanCartilage Fractures treatmentIDA  RGD 
Bmp4RatCartilage Fractures treatmentISOBMP4 (Homo sapiens) RGD 
Bmp4MouseCartilage Fractures treatmentISOBMP4 (Homo sapiens) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Bmp4  (bone morphogenetic protein 4)

Genes (Mus musculus)
Bmp4  (bone morphogenetic protein 4)

Genes (Homo sapiens)
BMP4  (bone morphogenetic protein 4)


Additional Information