RGD Reference Report - Different levels of TIMPs and MMPs in human lateral and medial rectus muscle tissue excised from strabismic patients. - Rat Genome Database

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Different levels of TIMPs and MMPs in human lateral and medial rectus muscle tissue excised from strabismic patients.

Authors: Kitada, M  Matsuo, T  Yamane, T  Hasebe, S  Ohtsuki, H 
Citation: Kitada M, etal., Strabismus. 2003 Sep;11(3):145-55.
RGD ID: 8699500
Pubmed: PMID:14710472   (View Abstract at PubMed)

PURPOSE: To arrive at an understanding of the differences in gene and protein expression between human lateral and medial rectus muscle tissue excised from strabismic patients. METHODS: Resected fragments of the lateral and medial rectus muscles obtained during strabismus surgery were frozen under liquid nitrogen and pulverized by a Freezer/Mill system. Radiolabelled cDNA was synthesized from polyA+ RNA and hybridized with a gene array nylon membrane (Atlas Human 1.2 Array) to detect the difference in gene expression between the lateral and medial rectus muscles. Based on the gene array results, tissue inhibitor of metalloproteinases (TIMP)-1 and 2, matrix metalloproteinase (MMP)-2, and bone morphogenetic protein (BMP)-4 were measured in solubilized muscle tissues by enzyme immunoassay and normalized by unit length of resection. RESULTS: The resected lateral rectus muscle had significantly smaller amounts of TIMP-1, TIMP-2 and BMP-4 but larger amounts of MMP-2 compared to the medial rectus muscle (P < 0.001, Mann-Whitney U test). The amounts of TIMP-1 in the muscle when the lateral and medial rectus were combined were positively correlated with TIMP-2 and BMP-4, while the amounts of TIMP-2 positively correlated with BMP-4 (P < 0.001, Spearman rank correlation test). The amounts of BMP-4 in both muscles increased significantly with age (P = 0.0003). CONCLUSIONS: Resected tissue from the lateral and medial rectus muscles had different levels of expression of TIMP-1 and 2, MMP-2, and BMP-4. These molecular differences may underlie different characteristics of the two extraocular muscles and may also influence the process of wound healing after strabismus surgery.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
BMP4Humanstrabismus  IEP  RGD 
Bmp4Ratstrabismus  ISOBMP4 (Homo sapiens) RGD 
Bmp4Mousestrabismus  ISOBMP4 (Homo sapiens) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Bmp4  (bone morphogenetic protein 4)

Genes (Mus musculus)
Bmp4  (bone morphogenetic protein 4)

Genes (Homo sapiens)
BMP4  (bone morphogenetic protein 4)


Additional Information