RGD Reference Report - Decreased expression of caveolin 1 in patients with systemic sclerosis: crucial role in the pathogenesis of tissue fibrosis. - Rat Genome Database

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Decreased expression of caveolin 1 in patients with systemic sclerosis: crucial role in the pathogenesis of tissue fibrosis.

Authors: Del Galdo, F  Sotgia, F  De Almeida, CJ  Jasmin, JF  Musick, M  Lisanti, MP  Jimenez, SA 
Citation: Del Galdo F, etal., Arthritis Rheum. 2008 Sep;58(9):2854-65. doi: 10.1002/art.23791.
RGD ID: 8661773
Pubmed: PMID:18759267   (View Abstract at PubMed)
PMCID: PMC2770094   (View Article at PubMed Central)
DOI: DOI:10.1002/art.23791   (Journal Full-text)

OBJECTIVE: Recent studies have implicated caveolin 1 in the regulation of transforming growth factor beta (TGFbeta) downstream signaling. Given the crucial role of TGFbeta in the pathogenesis of systemic sclerosis (SSc), we sought to determine whether caveolin 1 is also involved in the pathogenesis of tissue fibrosis in SSc. We analyzed the expression of CAV1 in affected SSc tissues, studied the effects of lack of expression of CAV1 in vitro and in vivo, and analyzed the effects of restoration of caveolin 1 function on the fibrotic phenotype of SSc fibroblasts in vitro. METHODS: CAV1 expression in tissues was analyzed by immunofluorescence and confocal microscopy. The extent of tissue fibrosis in Cav1-knockout mice was assessed by histologic/histochemical analyses and quantified by hydroxyproline assays. Cav1-null and SSc fibroblast phenotypes and protein production were analyzed by real-time polymerase chain reaction, immunofluorescence, Western blot, and multiplexed enzyme-linked immunosorbent assay techniques. The effects of restoration of caveolin 1 function in SSc fibroblasts in vitro were also examined using a cell-permeable recombinant CAV1 peptide. RESULTS: CAV1 was markedly decreased in the affected lungs and skin of SSc patients. Cav1-knockout mice developed pulmonary and skin fibrosis. Down-regulation of caveolin 1 was maintained in cultured SSc fibroblasts, and restoration of caveolin 1 function in vitro normalized their phenotype and abrogated TGFbeta stimulation through inhibition of Smad3 activation. CONCLUSION: Caveolin 1 appears to participate in the pathogenesis of tissue fibrosis in SSc. Restoration of caveolin 1 function by treatment with a cell-permeable peptide corresponding to the CAV1 scaffolding domain may be a novel therapeutic approach in SSc.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CAV1HumanDermal Fibrosis  ISOCav1 (Mus musculus) RGD 
Cav1RatDermal Fibrosis  ISOCav1 (Mus musculus) RGD 
Cav1MouseDermal Fibrosis  IMP  RGD 
CAV1Humanpulmonary fibrosis  ISOCav1 (Mus musculus) RGD 
Cav1Ratpulmonary fibrosis  ISOCav1 (Mus musculus) RGD 
Cav1Mousepulmonary fibrosis  IMP  RGD 
CAV1Humansystemic scleroderma  IEP protein:decreased expression:lung and skinRGD 
Cav1Ratsystemic scleroderma  ISOCAV1 (Homo sapiens)protein:decreased expression:lung and skinRGD 
Cav1Mousesystemic scleroderma  ISOCAV1 (Homo sapiens)protein:decreased expression:lung and skinRGD 

Objects Annotated

Genes (Rattus norvegicus)
Cav1  (caveolin 1)

Genes (Mus musculus)
Cav1  (caveolin 1, caveolae protein)

Genes (Homo sapiens)
CAV1  (caveolin 1)


Additional Information