RGD Reference Report - Vitamin A supplementation ameliorates obesity-associated retinal degeneration in WNIN/Ob rats. - Rat Genome Database

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Vitamin A supplementation ameliorates obesity-associated retinal degeneration in WNIN/Ob rats.

Authors: Tiruvalluru, M  Ananthathmakula, P  Ayyalasomayajula, V  Nappanveettil, G  Ayyagari, R  Reddy, GB 
Citation: Tiruvalluru M, etal., Nutrition. 2013 Jan;29(1):298-304. doi: 10.1016/j.nut.2012.06.006. Epub 2012 Oct 2.
RGD ID: 8657053
Pubmed: PMID:23036575   (View Abstract at PubMed)
DOI: DOI:10.1016/j.nut.2012.06.006   (Journal Full-text)

OBJECTIVE: Obesity is associated with various health afflictions, including ocular complications such as diabetic retinopathy, high intraocular pressure, cataracts, and macular degeneration. We previously reported progressive retinal degeneration after the onset of obesity in the spontaneously obese rat (WNIN/Ob) model. In the present study, we investigated vitamin A supplementation to ameliorate obesity-associated retinal degeneration in the WNIN/Ob rat. METHODS: Five-month-old male WNIN/Ob obese (O) and lean (L) control rats were fed with vitamin A 2.6 mg (L/O-I), 26 mg (L/O-II), 52 mg (L/O-III), and 129 mg (L/O-IV) per kilogram of diet as retinyl palmitate for 4 mo 2 wk. Retinal morphology and retinal gene expression were assessed by histologic, immunohistochemical, and real-time polymerase chain reaction methods. RESULTS: Supplementation of vitamin A at 26 or 52 mg significantly modulated the expression of retinal genes in the O but not in the L phenotype. Vitamin A supplementation significantly upregulated the expression of genes, such as rhodopsin, rod arrestin, phosphodiesterase, transducins, and fatty acid elongase-4, that were otherwise downregulated in O rat retina. The expression of glial fibrillary acidic protein was downregulated by vitamin A feeding in O rat retina. The immunohistochemical and histologic findings corroborated the gene expression data. The effects were significant at a 26- or 52-mg dose of vitamin A. CONCLUSION: Vitamin A supplementation alleviated obesity-associated retinal degeneration in the WNIN/Ob rat.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
W-Lepr+/+/NinRatretinal degeneration MODEL: controlIAGP compared to W-Leprfa/NinRGD 
W-LeprfaNinRatretinal degeneration MODEL: spontaneousIAGP compared to W-Lepr+/+/NinRGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
W-LeprfaNinRatabnormal photoreceptor outer segment morphology treatmentIAGPvitamin A RGD 
W-LeprfaNinRatabnormal retinol level  IAGP compared to W-Lepr+/+/NinRGD 
W-LeprfaNinRatdecreased body weight inducedIAGPvitamin A RGD 
W-LeprfaNinRatincreased circulating glucose level  IAGP compared to W-Lepr+/+/NinRGD 
W-LeprfaNinRatthin retina outer nuclear layer treatmentIAGPvitamin A RGD 

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Objects Annotated

Strains
W-Lepr+/+/Nin  (Wistar NIN lean)
W-LeprfaNin  (NA)


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