RGD Reference Report - Mechanical injury increases bFGF and CNTF mRNA expression in the mouse retina. - Rat Genome Database

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Mechanical injury increases bFGF and CNTF mRNA expression in the mouse retina.

Authors: Cao, W  Wen, R  Li, F  LaVail, MM  Steinberg, RH 
Citation: Cao W, etal., Exp Eye Res. 1997 Aug;65(2):241-8.
RGD ID: 8655567
Pubmed: PMID:9268592   (View Abstract at PubMed)
DOI: DOI:10.1006/exer.1997.0328   (Journal Full-text)

We characterized the survival-factor response of the normal mouse retina to mechanical injury by examining the expression of mRNAs for basic fibroblast growth factor (bFGF), ciliary neurotrophic factor (CNTF), and their receptors, FGF receptor-1 (FGFR-1) and CNTF receptor alpha (CNTFR-alpha). The retina was injured by making an incision through the choroid and retinal pigment epithelium that penetrated the subretinal space of each eye of an adult BALB/c mouse. Retinas were taken 6 hr, 12 hr, 1, 2, 4, 7, 10 and 16 days post-injury. Control animals were without injury. Northern blot analysis was performed to determine bFGF, CNTF and their receptor mRNA levels. A significant increase in bFGF and CNTF mRNAs was observed after injury, along with an increase in glial fibrillary acidic protein (GFAP) expression. More than 2-fold of upregulation of bFGF mRNA was seen as early as 6 hr after injury. This increase reached a maximum of more than 5-fold at day 2 post-injury and then declined slowly, and was still about 2.5-fold of the control level by day 16. Expression of CNTF showed a small increase of about 1.6-fold at 6 hr after injury. The upregulation reached a peak level of about 2.7-fold at day 4 after injury, then declined to control level by day 16. There was only a very small increase in FGFR-1 at 6, 12 and 24 hr after injury, and no significant increases in FGFR-1 at time points longer than 1 day post-injury. Expression of GFAP followed a time course similar to that of bFGF. We conclude that mechanical injury induces bFGF, CNTF, and GFAP expression in the mouse retina with time courses similar to the upregulation of these molecules in rat retina. Compared to the upregulation in rat retina, however, the injury-induced upregulation of bFGF and GFAP is much less in the mouse retina. In addition, there was only a very small induction of FGFR-1 expression in the mouse retina. These findings may explain, at least in part, the lack of injury-induced photoreceptor protection in the mouse retina.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FGF2HumanPenetrating Eye Injuries  ISOFgf2 (Mus musculus)mRNA:increased expression:retinaRGD 
Fgf2RatPenetrating Eye Injuries  ISOFgf2 (Mus musculus)mRNA:increased expression:retinaRGD 
Fgf2MousePenetrating Eye Injuries  IEP mRNA:increased expression:retinaRGD 

Objects Annotated

Genes (Rattus norvegicus)
Fgf2  (fibroblast growth factor 2)

Genes (Mus musculus)
Fgf2  (fibroblast growth factor 2)

Genes (Homo sapiens)
FGF2  (fibroblast growth factor 2)


Additional Information