RGD Reference Report - Association of inhibitory tyrosine protein kinase p50csk with protein tyrosine phosphatase PEP in T cells and other hemopoietic cells. - Rat Genome Database

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Association of inhibitory tyrosine protein kinase p50csk with protein tyrosine phosphatase PEP in T cells and other hemopoietic cells.

Authors: Cloutier, JF  Veillette, A 
Citation: Cloutier JF and Veillette A, EMBO J. 1996 Sep 16;15(18):4909-18.
RGD ID: 8554597
Pubmed: PMID:8890164   (View Abstract at PubMed)
PMCID: PMC452228   (View Article at PubMed Central)

p50csk is a tyrosine protein kinase (TPK) that represses the activity of Src family TPKs. We previously showed that Csk is a potent negative regulator of antigen receptor signaling in T lymphocytes and that its Src homology (SH) 3 and SH2 domains are required to inhibit these signals. To test the idea that the Csk SH3 and SH2 domains mediate interactions with other cellular proteins, we attempted to identify Csk-associated polypeptides using the yeast two-hybrid system. The results of our experiments demonstrated that Csk physically associates with PEP, a protein tyrosine phosphatase (PTP) expressed in hemopoietic cells. Further analyses revealed that this interaction was mediated by the Csk SH3 domain and by a proline-rich region (PPPLPERTP) in the non-catalytic C-terminal portion of PEP. The association between Csk and PEP was documented in transiently transfected Cos-1 cells and in a variety of cells of hemopoietic lineages, including T cells. Additional analyses demonstrated that the association between Csk and PEP is highly specific. Together, these data indicated that PEP may be an effector and/or a regulator of p50csk in T cells and other hemopoietic cells. Moreover, they allowed the identification of PEP as the first known ligand for the Csk SH3 domain.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CskRatproline-rich region binding enablesIPIUniProtKB:P29352PMID:8890164BHF-UCL 
CskRatprotein phosphatase binding enablesIPIUniProtKB:P29352PMID:8890164BHF-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Csk  (C-terminal Src kinase)


Additional Information