RGD Reference Report - Shootin1: A protein involved in the organization of an asymmetric signal for neuronal polarization. - Rat Genome Database

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Shootin1: A protein involved in the organization of an asymmetric signal for neuronal polarization.

Authors: Toriyama, M  Shimada, T  Kim, KB  Mitsuba, M  Nomura, E  Katsuta, K  Sakumura, Y  Roepstorff, P  Inagaki, N 
Citation: Toriyama M, etal., J Cell Biol. 2006 Oct 9;175(1):147-57.
RGD ID: 8554246
Pubmed: PMID:17030985   (View Abstract at PubMed)
PMCID: PMC2064506   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.200604160   (Journal Full-text)

Neurons have the remarkable ability to polarize even in symmetrical in vitro environments. Although recent studies have shown that asymmetric intracellular signals can induce neuronal polarization, it remains unclear how these polarized signals are organized without asymmetric cues. We describe a novel protein, named shootin1, that became up-regulated during polarization of hippocampal neurons and began fluctuating accumulation among multiple neurites. Eventually, shootin1 accumulated asymmetrically in a single neurite, which led to axon induction for polarization. Disturbing the asymmetric organization of shootin1 by excess shootin1 disrupted polarization, whereas repressing shootin1 expression inhibited polarization. Overexpression and RNA interference data suggest that shootin1 is required for spatially localized phosphoinositide-3-kinase activity. Shootin1 was transported anterogradely to the growth cones and diffused back to the soma; inhibiting this transport prevented its asymmetric accumulation in neurons. We propose that shootin1 is involved in the generation of internal asymmetric signals required for neuronal polarization.

Gene Ontology Annotations    

Biological Process
axonogenesis  (IDA)

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Pik3r1  (phosphoinositide-3-kinase regulatory subunit 1)
Shtn1  (shootin 1)

Additional Information