RGD Reference Report - Functional proteomics identify cornichon proteins as auxiliary subunits of AMPA receptors. - Rat Genome Database

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Functional proteomics identify cornichon proteins as auxiliary subunits of AMPA receptors.

Authors: Schwenk, J  Harmel, N  Zolles, G  Bildl, W  Kulik, A  Heimrich, B  Chisaka, O  Jonas, P  Schulte, U  Fakler, B  Klocker, N 
Citation: Schwenk J, etal., Science. 2009 Mar 6;323(5919):1313-9. doi: 10.1126/science.1167852.
RGD ID: 8554136
Pubmed: PMID:19265014   (View Abstract at PubMed)
DOI: DOI:10.1126/science.1167852   (Journal Full-text)

Glutamate receptors of the AMPA-subtype (AMPARs), together with the transmembrane AMPAR regulatory proteins (TARPs), mediate fast excitatory synaptic transmission in the mammalian brain. Here, we show by proteomic analysis that the majority of AMPARs in the rat brain are coassembled with two members of the cornichon family of transmembrane proteins, rather than with the TARPs. Coassembly with cornichon homologs 2 and 3 affects AMPARs in two ways: Cornichons increase surface expression of AMPARs, and they alter channel gating by markedly slowing deactivation and desensitization kinetics. These results demonstrate that cornichons are intrinsic auxiliary subunits of native AMPARs and provide previously unknown molecular determinants for glutamatergic neurotransmission in the central nervous system.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cnih2Ratlocalization within membrane acts_upstream_of_or_withinIDA PMID:19265014MGI 
Cacng2Ratregulation of AMPA receptor activity involved_inIDA PMID:19265014UniProt 
Cnih2Ratregulation of AMPA receptor activity involved_inIDA PMID:19265014UniProt 
Cnih3Ratregulation of AMPA receptor activity involved_inIDA PMID:19265014UniProt 
Cnih2Ratregulation of membrane potential acts_upstream_of_or_withinIDA PMID:19265014MGI 
Cnih3Ratregulation of membrane potential acts_upstream_of_or_withinIDA PMID:19265014MGI 
Cnih2Ratsynaptic transmission, glutamatergic acts_upstream_of_or_withinIDA PMID:19265014MGI 
Cnih3Ratsynaptic transmission, glutamatergic acts_upstream_of_or_withinIDA PMID:19265014MGI 

Cellular Component

  

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gria1RatAMPA glutamate receptor activity enablesIDA PMID:19265014UniProt 
Gria2RatAMPA glutamate receptor activity enablesIDA PMID:19265014UniProt 
Cnih3Ratchannel regulator activity enablesIDA PMID:19265014MGI 

Objects Annotated

Genes (Rattus norvegicus)
Cacng2  (calcium voltage-gated channel auxiliary subunit gamma 2)
Cacng3  (calcium voltage-gated channel auxiliary subunit gamma 3)
Cacng4  (calcium voltage-gated channel auxiliary subunit gamma 4)
Cacng5  (calcium voltage-gated channel auxiliary subunit gamma 5)
Cacng7  (calcium voltage-gated channel auxiliary subunit gamma 7)
Cacng8  (calcium voltage-gated channel auxiliary subunit gamma 8)
Cnih2  (cornichon family AMPA receptor auxiliary protein 2)
Cnih3  (cornichon family AMPA receptor auxiliary protein 3)
Gria1  (glutamate ionotropic receptor AMPA type subunit 1)
Gria2  (glutamate ionotropic receptor AMPA type subunit 2)
Gria3  (glutamate ionotropic receptor AMPA type subunit 3)
Gria4  (glutamate ionotropic receptor AMPA type subunit 4)


Additional Information