RGD Reference Report - PSD-95 is required to sustain the molecular organization of the postsynaptic density. - Rat Genome Database

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PSD-95 is required to sustain the molecular organization of the postsynaptic density.

Authors: Chen, X  Nelson, CD  Li, X  Winters, CA  Azzam, R  Sousa, AA  Leapman, RD  Gainer, H  Sheng, M  Reese, TS 
Citation: Chen X, etal., J Neurosci. 2011 Apr 27;31(17):6329-38. doi: 10.1523/JNEUROSCI.5968-10.2011.
RGD ID: 8554095
Pubmed: PMID:21525273   (View Abstract at PubMed)
PMCID: PMC3099547   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.5968-10.2011   (Journal Full-text)

PSD-95, a membrane-associated guanylate kinase, is the major scaffolding protein in the excitatory postsynaptic density (PSD) and a potent regulator of synaptic strength. Here we show that PSD-95 is in an extended configuration and positioned into regular arrays of vertical filaments that contact both glutamate receptors and orthogonal horizontal elements layered deep inside the PSD in rat hippocampal spine synapses. RNA interference knockdown of PSD-95 leads to loss of entire patches of PSD material, and electron microscopy tomography shows that the patchy loss correlates with loss of PSD-95-containing vertical filaments, horizontal elements associated with the vertical filaments, and putative AMPA receptor-type, but not NMDA receptor-type, structures. These observations show that the orthogonal molecular scaffold constructed from PSD-95-containing vertical filaments and their associated horizontal elements is essential for sustaining the three-dimensional molecular organization of the PSD. Our findings provide a structural basis for understanding the functional role of PSD-95 at the PSD.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Dlg4RatAMPA glutamate receptor clustering involved_inIMP PMID:21525273BHF-UCL 
Dlg4Ratdendritic spine morphogenesis involved_inIMP PMID:21525273BHF-UCL 
Dlg4Ratreceptor localization to synapse involved_inIMP PMID:21525273BHF-UCL 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Dlg4Ratglutamatergic synapse is_active_inIDA PMID:21525273SynGO 
Dlg4Ratglutamatergic synapse is_active_inIMP PMID:21525273SynGO 
Dlg4Ratpostsynaptic density located_inIDA PMID:21525273BHF-UCL 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Dlg4Ratstructural constituent of postsynaptic density enablesIDA PMID:21525273SynGO 
Dlg4Ratstructural constituent of postsynaptic density enablesIMP PMID:21525273SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Dlg4  (discs large MAGUK scaffold protein 4)


Additional Information