RGD Reference Report - Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex.

Advanced Search (OLGA)

General

Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex.
Authors:	Guan, T Muller, S Klier, G Pante, N Blevitt, JM Haner, M Paschal, B Aebi, U Gerace, L
Citation:	Guan T, etal., Mol Biol Cell. 1995 Nov;6(11):1591-603.
RGD ID:	8553994
Pubmed:	PMID:8589458 (View Abstract at PubMed)
PMCID:	PMC301313 (View Article at PubMed Central)
The p62 complex is an oligomeric assembly of O-linked glycoproteins of the nuclear pore complex that interacts with cytosolic transport factors and is part of the machinery for nuclear protein import. In this study we have purified the p62 complex from rat liver nuclear envelopes and analyzed its structure and composition. The p62 complex consists of four distinct polypeptides (p62, p58, p54, and p45) and has a mass of approximately 234 kDa, calculated from its hydrodynamic properties and supported by chemical cross-linking and scanning transmission electron microscopy. These data suggest that the p62 complex contains one copy of each constituent polypeptide. Analysis of preparations of the p62 complex by electron microscopy using rotary metal shadowing and negative staining revealed donut-shaped particles with a diameter of approximately 15 nm. Immunogold electron microscopy of isolated rat liver nuclear envelopes demonstrated that p62 occurs on both the nucleoplasmic and cytoplasmic sides of the pore complex near the central gated channel involved in active transport of proteins and RNAs. The properties and localization of the p62 complex suggest that it may be involved in binding transport ligands near the center of the nuclear pore complex and in subsequently transferring them to the gated transport channel.

Annotation Click to see Annotation Detail View

Gene Ontology Annotations Click to see Annotation Detail View

Cellular Component

Only show annotations with direct experimental evidence (0 objects hidden)

Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Nup54	Rat	nuclear pore	part_of	IDA		PMID:8589458	GO_Central
Nup58	Rat	nuclear pore	part_of	IDA		PMID:8589458	GO_Central
Nup62	Rat	nuclear pore	part_of	IDA		PMID:8589458	GO_Central

Molecular Function

Only show annotations with direct experimental evidence (0 objects hidden)

Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Nup54	Rat	structural constituent of nuclear pore	enables	IPI	UniProtKB:P17955	PMID:8589458	GO_Central
Nup58	Rat	structural constituent of nuclear pore	enables	IDA		PMID:8589458	GO_Central
Nup62	Rat	structural constituent of nuclear pore	enables	IDA		PMID:8589458	GO_Central

Objects Annotated

Genes (Rattus norvegicus)

Nup54 (nucleoporin 54)

Nup58 (nucleoporin 58)

Nup62 (nucleoporin 62)

Additional Information

Gene Annotator (Functional Annotation) unavailable	Gene Annotator (Annotation Distribution) unavailable	Variant Visualizer (Genomic Variants) unavailble Variant Visualizer (Genomic Variants) unavailable
Gene Annotator (Functional Annotation)	Gene Annotator (Annotation Distribution)	Variant Visualizer (Genomic Variants)

InterViewer (Protein-Protein Interactions) unavailable	Gviewer (Genome Viewer) unavailable	Variant Visualizer (Damaging Variants) unavailble Variant Visualizer (Damaging Variants) unavailable
InterViewer (Protein-Protein Interactions)	GViewer (Genome Viewer)	Variant Visualizer (Damaging Variants)

Gene Annotator (Annotation Comparison) unavailable	OLGA (Gene List Generator) unavailable
Gene Annotator (Annotation Comparison)	OLGA (Gene List Generator)	Excel (Download)

MOET (Multi-Ontology Enrichement) unavailable	GOLF (Gene-Ortholog Location Finder) unavailable
MOET (Multi-Ontology Enrichement)	GOLF (Gene-Ortholog Location Finder)

Create Name:

Description:

Send us a Message

Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex.