RGD Reference Report - CPEB2-eEF2 interaction impedes HIF-1alpha RNA translation. - Rat Genome Database

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CPEB2-eEF2 interaction impedes HIF-1alpha RNA translation.

Authors: Chen, PJ  Huang, YS 
Citation: Chen PJ and Huang YS, EMBO J. 2012 Feb 15;31(4):959-71. doi: 10.1038/emboj.2011.448. Epub 2011 Dec 9.
RGD ID: 8553654
Pubmed: PMID:22157746   (View Abstract at PubMed)
PMCID: PMC3280548   (View Article at PubMed Central)
DOI: DOI:10.1038/emboj.2011.448   (Journal Full-text)

Translation of mRNA into protein proceeds in three phases: initiation, elongation, and termination. Regulated translation allows the prompt production of selective proteins in response to physiological needs and is often controlled by sequence-specific RNA-binding proteins that function at initiation. Whether the elongation phase of translation can be modulated individually by trans-acting factors to synthesize polypeptides at variable rates remains to be determined. Here, we demonstrate that the RNA-binding protein, cytoplasmic polyadenylation element binding protein (CPEB)2, interacts with the elongation factor, eEF2, to reduce eEF2/ribosome-triggered GTP hydrolysis in vitro and slow down peptide elongation of CPEB2-bound RNA in vivo. The interaction of CPEB2 with eEF2 downregulates HIF-1alpha RNA translation under normoxic conditions; however, when cells encounter oxidative stress, CPEB2 dissociates from HIF-1alpha RNA, leading to rapid synthesis of HIF-1alpha for hypoxic adaptation. This study delineates the molecular mechanism of CPEB2-repressed translation and presents a unique model for controlling transcript-selective translation at elongation.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cpeb2Ratnegative regulation of cytoplasmic translational elongation involved_inIDA PMID:22157746UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cpeb2Ratcytoplasm located_inIDA PMID:22157746UniProt 
Cpeb2Ratnucleus located_inIDA PMID:22157746UniProt 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Cpeb2  (cytoplasmic polyadenylation element binding protein 2)


Additional Information