RGD Reference Report - Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export. - Rat Genome Database

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Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export.

Authors: Vasu, S  Shah, S  Orjalo, A  Park, M  Fischer, WH  Forbes, DJ 
Citation: Vasu S, etal., J Cell Biol. 2001 Oct 29;155(3):339-54. Epub 2001 Oct 29.
RGD ID: 8553287
Pubmed: PMID:11684705   (View Abstract at PubMed)
PMCID: PMC2150853   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.200108007   (Journal Full-text)

RNA undergoing nuclear export first encounters the basket of the nuclear pore. Two basket proteins, Nup98 and Nup153, are essential for mRNA export, but their molecular partners within the pore are largely unknown. Because the mechanism of RNA export will be in question as long as significant vertebrate pore proteins remain undiscovered, we set out to find their partners. Fragments of Nup98 and Nup153 were used for pulldown experiments from Xenopus egg extracts, which contain abundant disassembled nuclear pores. Strikingly, Nup98 and Nup153 each bound the same four large proteins. Purification and sequence analysis revealed that two are the known vertebrate nucleoporins, Nup96 and Nup107, whereas two mapped to ORFs of unknown function. The genes encoding the novel proteins were cloned, and antibodies were produced. Immunofluorescence reveals them to be new nucleoporins, designated Nup160 and Nup133, which are accessible on the basket side of the pore. Nucleoporins Nup160, Nup133, Nup107, and Nup96 exist as a complex in Xenopus egg extracts and in assembled pores, now termed the Nup160 complex. Sec13 is prominent in Nup98 and Nup153 pulldowns, and we find it to be a member of the Nup160 complex. We have mapped the sites that are required for binding the Nup160 subcomplex, and have found that in Nup98, the binding site is used to tether Nup98 to the nucleus; in Nup153, the binding site targets Nup153 to the nuclear pore. With transfection and in vivo transport assays, we find that specific Nup160 and Nup133 fragments block poly[A]+ RNA export, but not protein import or export. These results demonstrate that two novel vertebrate nucleoporins, Nup160 and Nup133, not only interact with Nup98 and Nup153, but themselves play a role in mRNA export.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nup107RatmRNA export from nucleus involved_inISSUniProtKB:P57740PMID:11684705UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nup107Ratnuclear pore part_ofISSUniProtKB:P57740PMID:11684705UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nup107Ratstructural constituent of nuclear pore enablesISSUniProtKB:P57740PMID:11684705UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Nup107  (nucleoporin 107)


Additional Information