RGD Reference Report - Expression of endothelial cell angiogenesis receptors in human cerebrovascular malformations. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Expression of endothelial cell angiogenesis receptors in human cerebrovascular malformations.

Authors: Uranishi, R  Baev, NI  Ng, PY  Kim, JH  Awad, IA 
Citation: Uranishi R, etal., Neurosurgery. 2001 Feb;48(2):359-67; discussion 367-8.
RGD ID: 8551824
Pubmed: PMID:11220380   (View Abstract at PubMed)

OBJECTIVE: To further understand the role of angiogenic growth factors in the development of cerebral cavernous malformations (CCMs) and arteriovenous malformations (AVMs), we investigated endothelial cell (EC) expression of receptors for vascular endothelial growth factor (VEGF) and angiopoietin systems in patients with surgically resected lesions. METHODS: Paraffin-embedded sections of five AVMs, CCMs, and normal control brain tissue samples were stained immunohistochemically with antibodies to von Willebrand factor and CD31 (to characterize ECs) and angiogenesis growth factor receptors Flt-1 (VEGF-R1), Flk-1 (VEGF-R2), Tie-1, and Tie-2. We counted large and small vessels in each specimen, assessed each specimen's immunoexpression of each antigen, and analyzed differences between CCMs, AVMs, and the normal control brain tissue samples. RESULTS: The ECs of CCMs, AVMs, and normal control brain tissue samples expressed the von Willebrand factor uniformly, but the ECs of CCMs were largely negative for CD31 (P < 0.05). Flk-1, Flt-1, and Tie-2 were not expressed in the control brain tissue samples. The proportion of immunopositive vessels to VEGF receptors Flk-1 and Flt-1 was significantly greater in AVMs and CCMs than in the control brain tissue samples (P < 0.05). Tie-2 in AVMs and CCMs was expressed in a higher percentage of immunopositive vessels than in the control brain tissue samples, but the difference was not statistically significant. Tie-1 was expressed in rare vessels of all lesion types and control brain tissue samples. CONCLUSION: ECs of CCMs do not seem to express CD31 to the same extent that AVMs and normal brain tissue do. AVMs and CCMs show greater expression of VEGF receptors, but not of angiopoietin receptors, than normal brain tissue does.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FLT1Humanarteriovenous malformations of the brain  IEP protein:increased expression:endothelial cell:RGD 
Flt1Mousearteriovenous malformations of the brain  ISOFLT1 (Homo sapiens)protein:increased expression:endothelial cell:RGD 
Flt1Ratarteriovenous malformations of the brain  ISOFLT1 (Homo sapiens)protein:increased expression:endothelial cell:RGD 
KDRHumanarteriovenous malformations of the brain  IEP protein:increased expression:endothelial cell:RGD 
KdrRatarteriovenous malformations of the brain  ISOKDR (Homo sapiens)protein:increased expression:endothelial cell:RGD 
KdrMousearteriovenous malformations of the brain  ISOKDR (Homo sapiens)protein:increased expression:endothelial cell:RGD 
FLT1Humancerebral cavernous malformation  IEP protein:increased expression:endothelial cell:RGD 
Flt1Ratcerebral cavernous malformation  ISOFLT1 (Homo sapiens)protein:increased expression:endothelial cell:RGD 
Flt1Mousecerebral cavernous malformation  ISOFLT1 (Homo sapiens)protein:increased expression:endothelial cell:RGD 
KDRHumancerebral cavernous malformation  IEP protein:increased expression:endothelial cell:RGD 
KdrRatcerebral cavernous malformation  ISOKDR (Homo sapiens)protein:increased expression:endothelial cell:RGD 
KdrMousecerebral cavernous malformation  ISOKDR (Homo sapiens)protein:increased expression:endothelial cell:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Flt1  (Fms related receptor tyrosine kinase 1)
Kdr  (kinase insert domain receptor)

Genes (Mus musculus)
Flt1  (FMS-like tyrosine kinase 1)
Kdr  (kinase insert domain protein receptor)

Genes (Homo sapiens)
FLT1  (fms related receptor tyrosine kinase 1)
KDR  (kinase insert domain receptor)


Additional Information