RGD Reference Report - Fms-like tyrosine kinase 3 ligand regulates migratory pattern and antigen uptake of lung dendritic cell subsets in a murine model of allergic airway inflammation. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Fms-like tyrosine kinase 3 ligand regulates migratory pattern and antigen uptake of lung dendritic cell subsets in a murine model of allergic airway inflammation.

Authors: Shao, Z  Makinde, TO  McGee, HS  Wang, X  Agrawal, DK 
Citation: Shao Z, etal., J Immunol. 2009 Dec 1;183(11):7531-8. doi: 10.4049/jimmunol.0901341. Epub 2009 Nov 16.
RGD ID: 8549811
Pubmed: PMID:19917684   (View Abstract at PubMed)
DOI: DOI:10.4049/jimmunol.0901341   (Journal Full-text)

Fms-like tyrosine kinase 3 ligand (Flt3L) reverses the features of allergic airway inflammation and increases a Th2-suppressive regulatory lung CD11c(high)CD11b(low) dendritic cell (DC) subset in a mouse model. We examined the migratory pattern and Ag uptake efficiency of lung DC subsets in the therapeutic effect of Flt3L. Lung CD11c(high)CD11b(low) and CD11c(low)CD11b(high) DCs from PBS-treated, OVA-sensitized, and Flt3L-treated/OVA-sensitized BALB/c mice were sorted using MACS and FACS for phenotype analysis. Lymphatic chemokine expression in thoracic lymph nodes was determined by immunohistochemistry. Migration of two lung DC subsets to lymphatic chemokines was examined in vitro using a Transwell chemotaxis assay. Labeled Ag was intranasally delivered into mouse lung to track the migration and Ag uptake of lung DCs. The in vitro cytokine secretion of mediastinal lymph node cells was determined using ELISA. CD11c(low)CD11b(high) DCs have higher expression of CCR5, CCR6, and CCR7, but lower expression of CCR2 than CD11c(high)CD11b(low) DCs. CD11c(low)CD11b(high) DCs in Flt3L-treated/OVA-sensitized mice demonstrated a less mature phenotype, inefficiency in Ag uptake, and impaired migration in vitro to lymphatic chemokine than those in OVA-sensitized mice. Administration of Flt3L decreased the expression of CCR5 and CCR7 in CD11c(low)CD11b(high) DCs in OVA-sensitized mice. Fewer Ag-carrying cells were detected in the lungs and lymph nodes in Flt3L-treated/OVA-sensitized mice than OVA-sensitized mice with a greater decrease in CD11c(low)CD11b(high) DCs. Mediastinal lymph node cells from Flt3L-treated mice secreted higher levels of Th1 cytokines and IL-10 than OVA-sensitized mice in vitro. In conclusion, Flt3L-generated lung immunogenic CD11c(low)CD11b(high) DCs have a less mature phenotype, impaired Ag uptake, and impaired migration to draining lymph nodes.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CCR2Humanasthma  ISOCcr2 (Mus musculus)protein:increased expression:lung and dendritic cell (mouse)RGD 
CCR6Humanasthma  ISOCcr6 (Mus musculus)protein:increased expression:lung and dendritic cell (mouse)RGD 
CCR7Humanasthma  ISOCcr7 (Mus musculus)protein:increased expression:lung and dendritic cell (mouse)RGD 
Ccr2Ratasthma  ISOCcr2 (Mus musculus)protein:increased expression:lung and dendritic cell (mouse)RGD 
Ccr2Mouseasthma  IEP protein:increased expression:lung and dendritic cell (mouse)RGD 
Ccr6Ratasthma  ISOCcr6 (Mus musculus)protein:increased expression:lung and dendritic cell (mouse)RGD 
Ccr6Mouseasthma  IEP protein:increased expression:lung and dendritic cell (mouse)RGD 
Ccr7Ratasthma  ISOCcr7 (Mus musculus)protein:increased expression:lung and dendritic cell (mouse)RGD 
Ccr7Mouseasthma  IEP protein:increased expression:lung and dendritic cell (mouse)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ccr2  (C-C motif chemokine receptor 2)
Ccr6  (C-C motif chemokine receptor 6)
Ccr7  (C-C motif chemokine receptor 7)

Genes (Mus musculus)
Ccr2  (C-C motif chemokine receptor 2)
Ccr6  (C-C motif chemokine receptor 6)
Ccr7  (C-C motif chemokine receptor 7)

Genes (Homo sapiens)
CCR2  (C-C motif chemokine receptor 2)
CCR6  (C-C motif chemokine receptor 6)
CCR7  (C-C motif chemokine receptor 7)


Additional Information