RGD Reference Report - Effects of amniotic membrane transplantation on cytokines expression in chemically burned rat corneas. - Rat Genome Database

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Effects of amniotic membrane transplantation on cytokines expression in chemically burned rat corneas.

Authors: Gu, HW  Bian, DM  Hu, N  Zhang, JF 
Citation: Gu HW, etal., Int J Ophthalmol. 2011;4(1):33-6. doi: 10.3980/j.issn.2222-3959.2011.01.07. Epub 2011 Feb 18.
RGD ID: 7364851
Pubmed: PMID:22553604   (View Abstract at PubMed)
PMCID: PMC3340686   (View Article at PubMed Central)
DOI: DOI:10.3980/j.issn.2222-3959.2011.01.07   (Journal Full-text)

AIM: To investigate the effect of amniotic membrane transplantation (AMT) on the expressions of inflammatory-related, angiogenic-related and growth-related cytokines in rat corneas after chemical injury. METHODS: Alkali wounds were inflicted on the central corneas of rats by applying a round filter paper soaked in 1mol/L NaOH for 40 seconds. One week after alkali burn, 12 rats were randomly divided into 2 groups: the AMT group and the control group, and AMT was performed on the rats in the AMT group. Corneal opacity and neovascularization were observed by slit-lamp microscopy. The protein levels of interleukin (IL)-2, interferon (IFN)-gamma, IL-10 and transforming growth factor (TGF)-beta were determined by enzyme-linked immunosorbent assay 2 weeks after AMT. The mRNA levels of matrix metalloproteinase-2 (MMP-2), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) were evaluated by real-time quantitative PCR. RESULTS: In the AMT group, the corneal opacity was improved (P=0.011) and the area of corneal neovascularization was significantly decreased (P=0.005) compared with the control group. The amount of IL-2 and IFN-gamma secreted by Th1 cells were decreased after AMT, whereas the amount of IL-10 and TGF-beta secreted by Th2 cells were increased (P<0.05). The level of MMP-2 was significantly down-regulated (P=0.013) at the mRNA level in the AMT group, while the expression of EGF was significantly higher (P=0.022) compared with controls. CONCLUTION: AMT may suppress corneal neovascularization after chemical injury by modulating the expressions of soluble factors.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IL10HumanEye Burns treatmentISOIl10 (Rattus norvegicus) RGD 
Il10RatEye Burns treatmentIDA  RGD 
Il10MouseEye Burns treatmentISOIl10 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il10  (interleukin 10)

Genes (Mus musculus)
Il10  (interleukin 10)

Genes (Homo sapiens)
IL10  (interleukin 10)


Additional Information