RGD Reference Report - Altered bladder function in transgenic mice expressing rat elastin. - Rat Genome Database

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Altered bladder function in transgenic mice expressing rat elastin.

Authors: Lemack, GE  Szabo, Z  Urban, Z  Boyd, CD  Csiszar, K  Vaughan ED, JR  Felsen, D 
Citation: Lemack GE, etal., Neurourol Urodyn 1999;18(1):55-68.
RGD ID: 731206
Pubmed: PMID:10090127   (View Abstract at PubMed)

The elasticity of tissues subjected to repeated deformation is provided by the presence of elastic fibers in the extracellular matrix (ECM). The most abundant component of elastic fibers is elastin, whose soluble precursor is tropoelastin. To establish the role elastin plays in the bladder, this study describes the biosynthetic, histologic, and physiologic consequences of expression of an isoform of rat tropoelastin in transgenic mouse bladder. The polymerase chain reaction (PCR) was used to determine expression of a rat tropoelastin minigene in transgenic mice. Histochemical methods were used to demonstrate changes in elastic fibers in frozen sections of bladder. Cystometric analysis was carried out in transgenic and non-transgenic mice, prior to and after 3 weeks of partial outlet obstruction. The PCR assay demonstrated that bladder tissue of transgenic mice expressed rat tropoelastin mRNA, whereas non-transgenes did not. Increased deposition of elastic fibers was demonstrated with the Verhoeff-van Gieson stain. Bladders of transgenic animals were more compliant than bladders of their non-transgenic littermates. Partial outlet obstruction resulted in increased bladder volume and more compliant bladders in non-transgenic mice. In contrast, the bladder volume and compliance in transgenes was almost unchanged by obstruction. This study demonstrates that normal elastic fiber assembly is prerequisite for the compliant properties of the bladder wall. Moreover, the response of the bladder to obstruction is critically influenced by elastin synthesis.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ElnRatelastic fiber assembly  TAS  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ElnRatextracellular matrix constituent conferring elasticity  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Eln  (elastin)

Objects referenced in this article
Gene Eln elastin Mus musculus

Additional Information