RGD Reference Report - Isolation and characterization of rat cDNA clones for two distinct thyroid hormone receptors. - Rat Genome Database

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Isolation and characterization of rat cDNA clones for two distinct thyroid hormone receptors.

Authors: Murray, MB  Zilz, ND  McCreary, NL  MacDonald, MJ  Towle, HC 
Citation: Murray MB, etal., J Biol Chem 1988 Sep 5;263(25):12770-7.
RGD ID: 729962
Pubmed: PMID:2457590   (View Abstract at PubMed)

Two distinct v-erbA-related cDNA clones representing the products of different genes were isolated from a rat liver cDNA library. The first, rc-erbA-alpha, was 82% identical to v-erbA and encoded a polypeptide with a calculated molecular mass of 45,000 daltons. This cDNA clone arises from the same gene product as a v-erbA-related cDNA isolated from rat brain by Thompson et al. (Thompson, C. C., Weinberger, C., Lebo, R., and Evans, R. (1987) Science 237, 1610-1614). The second cDNA clone, rc-erbA-beta, was 76% identical to v-erbA and encoded a polypeptide with a calculated molecular mass of 52,000 daltons. Both rc-erbA-alpha and rc-erbA-beta translational products bound 3,5,3'-triiodo-L-thyronine with affinities equal to each other (Kd approximately equal to 0.4 nM) and comparable to the nuclear thyroid hormone receptor extracted from rat liver. The relative affinities of a series of thyroid hormone analogs for both translational products were also identical. In various tissues and cell lines, the relative levels of rc-erbA-beta RNA, but not rc-erbA-alpha RNA, correlated with measurements of nuclear 3,5,3'-triiodo-L-thyronine binding sites. Based on this correlation, we suggest that rc-erbA-beta may encode the "classical" nuclear thyroid hormone receptor, whereas rc-erbA-alpha may encode an isoreceptor species with differing functional properties.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ThrbRatnuclear receptor activity  IDA  RGD 
ThrbRatnuclear receptor activity  TAS  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Thrb  (thyroid hormone receptor beta)


Additional Information