RGD Reference Report - Identification of a phospholipase C beta subtype in rat taste cells. - Rat Genome Database

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Identification of a phospholipase C beta subtype in rat taste cells.

Authors: Rossler, P  Kroner, C  Freitag, J  Noe, J  Breer, H 
Citation: Rossler P, etal., Eur J Cell Biol 1998 Nov;77(3):253-61.
RGD ID: 729588
Pubmed: PMID:9860142   (View Abstract at PubMed)

From rat circumvallate papillae a novel phospholipase C (PLC) subtype has been cloned and identified as most closely related to human PLC beta2. The corresponding mRNA was only detected in sensory lingual tissue but not in non-taste lingual tissue or any other tissues examined by Northern blot analysis. In situ hybridization revealed that a subset of taste receptor cells of circumvallate papillae was specifically labeled. A functional involvement of this PLC beta subtype in taste signal transduction emerged from biochemical analysis monitoring the second messenger response in circumvallate preparations induced by denatonium benzoate. This bitter agent elicited a rapid and transient increase of the inositol 1,4,5-trisphosphate level; this response was blocked by U73122, a potent inhibitor of PLC, and by PLC beta2-specific antibodies. These data indicate that a phospholipase C beta2 isoform mediates a denatonium benzoate-induced second messenger response of taste sensory cells in the circumvallate papillae.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Plcb2Ratphosphatidylinositol phospholipase C activity enablesIMP PMID:9860142UniProt 
Plcb2Ratphospholipase C activity  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Plcb2  (phospholipase C, beta 2)


Additional Information