RGD Reference Report - Cloning and expression of rat cDNA encoding corticosteroid 11 beta-dehydrogenase. - Rat Genome Database

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Cloning and expression of rat cDNA encoding corticosteroid 11 beta-dehydrogenase.

Authors: Agarwal, AK  Monder, C  Eckstein, B  White, PC 
Citation: Agarwal AK, etal., J Biol Chem 1989 Nov 15;264(32):18939-43.
RGD ID: 728825
Pubmed: PMID:2808402   (View Abstract at PubMed)

Corticosteroid 11 beta-dehydrogenase (11-DH) catalyzes the conversion of cortisol to the inactive metabolite cortisone. Absence of 11-DH activity leads to a potentially fatal form of childhood hypertension termed apparent mineralocorticoid excess. As a first step in elucidating the molecular basis of this disorder, we isolated and characterized a rat cDNA clone encoding 11-DH. This clone hybridized to a single mRNA species in liver, kidney, and testis RNA but not to RNA from heart. The insert was 1265 base pairs long and included an 861-base pair open reading frame encoding 287 amino acids. A search of sequence databases revealed that 11-DH is identical in about 27% of amino acid residues to ribitol dehydrogenase from Klebsiella and to the product of the nodG gene from the nitrogen-fixing bacterium, Rhizobium meliloti, thus defining a new superfamily of genes encoding dehydrogenases. The 11-DH cDNA was expressed by transfection into Chinese hamster ovary cells under the control of an SV40 promoter. The expressed enzyme mediated both 11 beta-dehydrogenation and the reverse 11-oxoreduction reaction. Southern blot analysis of rat and human DNA suggested that additional genes related to 11-DH exist in both species.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Hsd11b1Ratmineralocorticoid metabolic process  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hsd11b1  (hydroxysteroid 11-beta dehydrogenase 1)


Additional Information