RGD Reference Report - Production of knockout rats using ENU mutagenesis and a yeast-based screening assay. - Rat Genome Database

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Production of knockout rats using ENU mutagenesis and a yeast-based screening assay.

Authors: Zan, Y  Haag, JD  Chen, KS  Shepel, LA  Wigington, D  Wang, YR  Hu, R  Lopez-Guajardo, CC  Brose, HL  Porter, KI  Leonard, RA  Hitt, AA  Schommer, SL  Elegbede, AF  Gould, MN 
Citation: Zan Y, etal., Nat Biotechnol 2003 Jun;21(6):645-51.
RGD ID: 727990
Pubmed: (View Article at PubMed) PMID:12754522
DOI: Full-text: DOI:10.1038/nbt830

The rat is a widely used model in biomedical research and is often the preferred rodent model in many areas of physiological and pathobiological research. Although many genetic tools are available for the rat, methods to produce gene-disrupted knockout rats are greatly needed. In this study, we developed protocols for creating N-ethyl-N-nitrosourea (ENU)-induced germline mutations in several rat strains. F1 preweanling pups from mutagenized Sprague Dawley (SD) male rats were then screened for functional mutations in Brca1 and Brca2 using a yeast gap-repair, ADE2-reporter truncation assay. We produced knockout rats for each of these two breast cancer suppressor genes.



Disease Annotations    
atrophy of testis  (IMP,ISO)
infertility  (IMP,ISO)

Phenotype Annotations    

Mammalian Phenotype
Objects Annotated

Genes (Rattus norvegicus)
Brca2  (BRCA2, DNA repair associated)
Brca2m1Uwm  (BRCA2, DNA repair associated; mutation 1, University of Wisconsin-Madison)

Genes (Mus musculus)
Brca2  (breast cancer 2, early onset)

Genes (Homo sapiens)
BRCA2  (BRCA2 DNA repair associated)

Strains
SD-Brca2m1Uwm  (NA)

Objects referenced in this article
Gene Brca1 BRCA1, DNA repair associated Rattus norvegicus
Gene Brca1m1Uwm BRCA1, DNA repair associated; mutation 1, University of Wisconsin-Madison Rattus norvegicus
Strain SD-Brca1m1Uwm null Rattus norvegicus

Additional Information