RGD Reference Report - A novel type of regulation of the vimentin intermediate filament cytoskeleton by a Golgi protein. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

A novel type of regulation of the vimentin intermediate filament cytoskeleton by a Golgi protein.

Authors: Gao, YS  Vrielink, A  MacKenzie, R  Sztul, E 
Citation: Gao YS, etal., Eur J Cell Biol 2002 Jul;81(7):391-401.
RGD ID: 727402
Pubmed: PMID:12160147   (View Abstract at PubMed)
DOI: DOI:10.1078/0171-9335-00260   (Journal Full-text)

Whether the highly dynamic structure of the vimentin intermediate filament (IF) cytoskeleton responds to cues from cellular organelles, and what proteins might participate in such events is largely unknown. We have shown previously that the Golgi protein formiminotransferase cyclodeaminase (FTCD) binds to vimentin filaments in vivo and in vitro, and that overexpression of FTCD causes dramatic rearrangements of the vimentin IF cytoskeleton (Gao and Sztul, J. Cell Biol. 152, 877-894, 2001). Using real-time imaging, we now show that FTCD causes bundling of individual thinner vimentin filaments into fibers and that the bundling always originates at the Golgi. FTCD appears to be the molecular "glue" since FTCD cross-links vimentin filaments in vitro. To initiate the analysis of structural determinants required for FTCD function in vimentin dynamics, we used structure-based design to generate individual formiminotransferase (FT) and cyclodeaminase (CD) domains, and to produce an enzymatically inactive FTCD. We show that the intact octameric structure is required for FTCD binding to vimentin filaments and for promoting filament assembly, but that eliminating enzymatic activity does not affect FTCD effects on the vimentin cytoskeleton. Our findings indicate that the Golgi protein FTCD is a potent modulator of the vimentin IF cytoskeleton, and suggest that the Golgi might act as a reservoir for proteins that regulate cytoskeletal dynamics.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FtcdRatcytoskeleton organization  IMP  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FtcdRatformimidoyltetrahydrofolate cyclodeaminase activity  TAS  RGD 
FtcdRatglutamate formimidoyltransferase activity  TAS  RGD 
FtcdRatintermediate filament binding  TAS  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ftcd  (formimidoyltransferase cyclodeaminase)


Additional Information