RGD Reference Report - Crystal structure of the carbohydrate recognition domain of p58/ERGIC-53, a protein involved in glycoprotein export from the endoplasmic reticulum. - Rat Genome Database

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Crystal structure of the carbohydrate recognition domain of p58/ERGIC-53, a protein involved in glycoprotein export from the endoplasmic reticulum.

Authors: Velloso, LM  Svensson, K  Schneider, G  Pettersson, RF  Lindqvist, Y 
Citation: Velloso LM, etal., J Biol Chem 2002 May 3;277(18):15979-84.
RGD ID: 727344
Pubmed: PMID:11850423   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M112098200   (Journal Full-text)

p58/ERGIC-53 is an animal calcium-dependent lectin that cycles between the endoplasmic reticulum (ER) and the Golgi complex and appears to act as a cargo receptor for a subset of soluble glycoproteins exported from the ER. We have determined the crystal structure of the carbohydrate recognition domain (CRD) of p58, the rat homologue of human ERGIC-53, to 1.46 A resolution. The fold and ligand binding site are most similar to those of leguminous lectins. The structure also resembles that of the CRD of the ER folding chaperone calnexin and the neurexins, a family of non-lectin proteins expressed on neurons. The CRD comprises one concave and one convex beta-sheet packed into a beta-sandwich. The ligand binding site resides in a negatively charged cleft formed by conserved residues. A large surface patch of conserved residues with a putative role in protein-protein interactions and oligomerization lies on the opposite side of the ligand binding site. Together with previous functional data, the structure defines a new and expanding class of calcium-dependent animal lectins and provides a starting point for the understanding of glycoprotein sorting between the ER and the Golgi.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Lman1Ratendoplasmic reticulum-Golgi intermediate compartment  TAS  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Lman1Ratcalcium ion binding  TAS  RGD 
Lman1RatD-mannose binding  TAS  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lman1  (lectin, mannose-binding, 1)

Objects referenced in this article
Gene Lman1 lectin, mannose-binding, 1 Mus musculus
Gene Nr3c2 nuclear receptor subfamily 3, group C, member 2 Mus musculus

Additional Information