RGD Reference Report - Tracheal occlusion modulates the gene expression profile of the medial thalamus in anesthetized rats. - Rat Genome Database

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Tracheal occlusion modulates the gene expression profile of the medial thalamus in anesthetized rats.

Authors: Bernhardt, V  Garcia-Reyero, N  Vovk, A  Denslow, N  Davenport, PW 
Citation: Bernhardt V, etal., J Appl Physiol. 2011 Jul;111(1):117-24. doi: 10.1152/japplphysiol.01317.2010. Epub 2011 Apr 28.
RGD ID: 7248613
Pubmed: PMID:21527662   (View Abstract at PubMed)
PMCID: PMC6169110   (View Article at PubMed Central)
DOI: DOI:10.1152/japplphysiol.01317.2010   (Journal Full-text)

Conscious awareness of breathing requires the activation of higher brain centers and is believed to be a neural gated process. The thalamus could be responsible for the gating of respiratory sensory information to the cortex. It was reasoned that if the thalamus is the neural gate, then tracheal obstructions will modulate the gene expression profile of the thalamus. Anesthetized rats were instrumented with an inflatable cuff sutured around the trachea. The cuff was inflated to obstruct 2-4 breaths, then deflated for a minimum of 15 breaths. Obstructions were repeated for 10 min followed by immediate dissection of the medial thalamus. Following the occlusion protocol, 588 genes were found to be altered (P < 0.05; log(2) fold change >/= 0.4), with 327 genes downregulated and 261 genes upregulated. A significant upregulation of the serotonin HTR2A receptor and significant downregulation of the dopamine DRD1 receptor genes were found. A pathway analysis was performed that targeted serotonin and dopamine receptor pathways. The mitogen-activated protein kinase 1 (MAPK1) gene was significantly downregulated. MAPK1 is an inhibitory regulator of HTR2A and facilitatory regulator for DRD1. Downregulation of MAPK1 may be related to the significant upregulation of HTR2A and downregulation of DRD1, suggesting an interaction in the medial thalamus serotonin-dopamine pathway elicited by airway obstruction. These results demonstrate an immediate change in gene expression in thalamic arousal, fear, anxiety motivation-related serotonin and dopamine receptors in response to airway obstruction. The results support the hypothesis that the thalamus is a component in the respiratory mechanosensory neural pathway.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
DRD1Humantracheal stenosis  ISODrd1 (Rattus norvegicus)mRNA:decreased expression:medial dorsal nucleus of thalamus (rat)RGD 
Drd1Rattracheal stenosis  IEP mRNA:decreased expression:medial dorsal nucleus of thalamus (rat)RGD 
Drd1Mousetracheal stenosis  ISODrd1 (Rattus norvegicus)mRNA:decreased expression:medial dorsal nucleus of thalamus (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Drd1  (dopamine receptor D1)

Genes (Mus musculus)
Drd1  (dopamine receptor D1)

Genes (Homo sapiens)
DRD1  (dopamine receptor D1)


Additional Information