RGD Reference Report - Biochemical characterization of nuclear pore complex protein gp210 oligomers. - Rat Genome Database

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Biochemical characterization of nuclear pore complex protein gp210 oligomers.

Authors: Favreau, C  Bastos, R  Cartaud, J  Courvalin, JC  Mustonen, P 
Citation: Favreau C, etal., Eur J Biochem 2001 Jul;268(14):3883-9.
RGD ID: 724456
Pubmed: PMID:11453980   (View Abstract at PubMed)

The membrane-spanning glycoprotein gp210 is a major component of the nuclear pore complex. This nucleoporin contains a large cisternal N-terminal domain, a short C-terminal cytoplasmic tail, and a single transmembrane segment. We show here that dimers of native gp210 can be isolated from cell extracts by immunoprecipitation, and from purified rat liver nuclear envelopes by velocity sedimentation and gel filtration. Cross-linking of proteins in isolated membranes prior to solubilization dramatically increases the proportion of dimers. The dimers are SDS-resistant, as previously observed for some integral membrane proteins of cis-Golgi and plasma membrane proteins, including glycophorin A. Larger oligomers of gp210 can also be obtained by gel filtration and denaturing electrophoresis, but unlike the dimers are dissociated by reduction and heating in the presence of SDS. We propose that gp210 is organized into the pore membrane as a large array of gp210 dimers that may constitute a luminal submembranous protein skeleton.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nup210Ratprotein-containing complex assembly  IDA dimerizationRGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nup210Ratnuclear pore  TAS  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nup210Ratstructural constituent of nuclear pore  NAS  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nup210  (nucleoporin 210)


Additional Information