RGD Reference Report - Functional requirement of aquaporin-5 in plasma membranes of sweat glands. - Rat Genome Database

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Functional requirement of aquaporin-5 in plasma membranes of sweat glands.

Authors: Nejsum, LN  Kwon, TH  Jensen, UB  Fumagalli, O  Frokiaer, J  Krane, CM  Menon, AG  King, LS  Agre, PC  Nielsen, S 
Citation: Nejsum LN, etal., Proc Natl Acad Sci U S A 2002 Jan 8;99(1):511-516.
RGD ID: 70240
Pubmed: PMID:11773623   (View Abstract at PubMed)
PMCID: PMC117591   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.012588099   (Journal Full-text)

The distribution and function of aquaporins (AQPs) have not previously been defined in sweat glands. In this study, AQP1, AQP3, and AQP5 mRNA were demonstrated in rat paw by reverse transcription (RT)-PCR, but AQP2 and AQP4 were not. AQP1, AQP3, and AQP5 protein were confirmed in these tissues by immunoblotting. AQP1 was identified in capillary endothelial cells by immunohistochemical labeling, but not in sweat glands or epidermis. Abundant AQP3 expression was seen in basal levels of epidermis, but not in sweat glands. AQP2 and AQP4 were not observed in either skin or sweat glands. Immunohistochemical labeling revealed abundant AQP5 in secretory parts of rat and mouse sweat glands, where immunoelectron microscopy demonstrated abundant AQP5 labeling in the apical plasma membrane. AQP5 immunolabeling of human sweat glands yielded a similar pattern. To establish the role of AQP5 in sweat secretion, we tested the response of adult mice to s.c. injection of pilocarpine, as visualized by reaction of secreted amylase with iodine/starch. The number of active sweat glands was dramatically reduced in AQP5-null (-/-) mice compared with heterozygous (+/-) and wild-type (+/+) mice. We conclude that the presence of AQP5 in plasma membranes of sweat glands is essential for secretion, providing potential insight into mechanisms underlying mammalian thermoregulation, tactile sensitivity, and the pathophysiology of hyperhidrosis.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AQP5Humanhypohidrosis  ISOAqp5 (Mus musculus) RGD 
Aqp5Rathypohidrosis  ISOAqp5 (Mus musculus) RGD 
Aqp5Mousehypohidrosis  IMP  RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Aqp5Ratapical plasma membrane  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Aqp5Ratwater channel activity  TAS  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Aqp5  (aquaporin 5)

Genes (Mus musculus)
Aqp5  (aquaporin 5)

Genes (Homo sapiens)
AQP5  (aquaporin 5)

Objects referenced in this article
Gene Aqp1 aquaporin 1 Rattus norvegicus
Gene Aqp2 aquaporin 2 Rattus norvegicus
Gene Aqp3 aquaporin 3 (Gill blood group) Rattus norvegicus
Gene Aqp4 aquaporin 4 Rattus norvegicus

Additional Information