RGD Reference Report - Isolation and expression of rat liver sepiapterin reductase cDNA. - Rat Genome Database

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Isolation and expression of rat liver sepiapterin reductase cDNA.

Authors: Citron, BA  Milstien, S  Gutierrez, JC  Levine, RA  Yanak, BL  Kaufman, S 
Citation: Citron BA, etal., Proc Natl Acad Sci U S A 1990 Aug;87(16):6436-40.
RGD ID: 70022
Pubmed: PMID:2201030   (View Abstract at PubMed)
PMCID: PMC54549   (View Article at PubMed Central)

Sepiapterin reductase (7,8-dihydrobiopterin: NADP+ oxidoreductase, EC 1.1.1.153) catalyzes the terminal step in the biosynthetic pathway for tetrahydrobiopterin, the cofactor necessary for aromatic amino acid hydroxylation. We report here the isolation of a cDNA clone for rat liver sepiapterin reductase. The cDNA has been excised from a lambda vector and the DNA sequence was determined. The insert contains the coding sequence for at least 95% of the rat enzyme and is fused to the Escherichia coli beta-galactosidase N-terminal segment and the lac promoter. The N-terminal region of the clone contains an extraordinarily high G + C content. The amino acid sequence deduced from the clone is in agreement with the size and composition of the enzyme and was matched to several tryptic peptide sequences. The enzyme encoded by the cDNA insert was shown to have sepiapterin reductase activity after expression in E. coli. Structural similarities were identified between this protein and several enzymes that should contain similar nucleotide and pteridine binding sites.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SprRattetrahydrobiopterin biosynthetic process  TAS  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SprRatsepiapterin reductase (NADP+) activity  IDA  RGD 
SprRatsepiapterin reductase (NADP+) activity  TAS  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Spr  (sepiapterin reductase)


Additional Information