RGD Reference Report - Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney. - Rat Genome Database

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Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney.

Authors: Gamba, G  Miyanoshita, A  Lombardi, M  Lytton, J  Lee, WS  Hediger, MA  Hebert, SC 
Citation: Gamba G, etal., J Biol Chem 1994 Jul 1;269(26):17713-22.
RGD ID: 69999
Pubmed: PMID:8021284   (View Abstract at PubMed)

Electrically silent Na(+)-(K+)-Cl- transporter systems are present in a wide variety of cells and serve diverse physiological functions. In chloride secretory and absorbing epithelia, these cotransporters provide the chloride entry mechanism crucial for transcellular chloride transport. We have isolated cDNAs encoding the two major electroneutral sodium-chloride transporters present in the mammalian kidney, the bumetanide-sensitive Na(+)-K(+)-Cl- symporter and thiazide-sensitive Na(+)-Cl- cotransporter, and have characterized their functional activity in Xenopus laevis oocytes. Despite their differing sensitivities to bumetanide and thiazides and their different requirements for potassium, these approximately 115-kDa proteins share significant sequence similarity (approximately 60%) and exhibit a topology featuring 12 potential membrane-spanning helices flanked by long non-hydrophobic domains at the NH2 and COOH termini. Northern blot analysis and in situ hybridization indicate that these transporters are expressed predominantly in kidney with an intrarenal distribution consistent with their recognized functional localization. These proteins establish a new family of Na(+)-(K+)-Cl- cotransporters.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc12a3Ratchloride transport  IMP  RGD 
Slc12a3Ratsodium ion transport  IMP  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc12a3Ratsodium:chloride symporter activity  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc12a3  (solute carrier family 12 member 3)

Objects referenced in this article
Gene Slc12a1 solute carrier family 12, member 1 Mus musculus
Gene Slc12a3 solute carrier family 12, member 3 Mus musculus
Gene Slc12a1 solute carrier family 12 member 1 Rattus norvegicus

Additional Information