RGD Reference Report - Cloning and gene structure of rat phosphatidylcholine transfer protein, Pctp. - Rat Genome Database

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Cloning and gene structure of rat phosphatidylcholine transfer protein, Pctp.

Authors: Wu, MK  Boylan, MO  Cohen, DE 
Citation: Wu MK, etal., Gene 1999 Jul 22;235(1-2):111-20.
RGD ID: 69923
Pubmed: PMID:10415339   (View Abstract at PubMed)

Phosphatidylcholine transfer protein (PC-TP) is a cytosolic lipid transfer protein that promotes intermembrane transfer of phosphatidylcholines but no other phospholipids. Although its physiological function remains unknown, phosphatidylcholine transfer protein is enriched in liver and evidence from model systems suggests a role in hepatocellular selection and transport of biliary phospholipids. To facilitate in vivo studies, a cDNA encoding rat PC-TP was cloned by library screening and 5'-rapid amplification of cDNA ends. Genomic cloning demonstrated the rat Pctp gene spans 10. 8kb and is comprised of six exons. The putative transcription initiation site was identified 50bp upstream of the translation initiation site. Nucleotide sequence analysis of the 5'-flanking region revealed a CAAT- but no TATA-box. Transient transfection of a series of 5'-deleted Pctp-promoter-firefly luciferase constructs into Reuber H35 rat hepatoma cells, which express Pctp mRNA, and Gunn rat fibroblasts, which do not, suggest that cis-acting elements in a 637bp promoter region contribute to enhanced expression of PC-TP in liver.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
phospholipid transport  TAS 69923 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
phosphatidylcholine transporter activity  TAS 69923 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pctp  (phosphatidylcholine transfer protein)

Additional Information